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Dr. Danny Llewellyn, CSIRO, discusses some of the key biotechnology projects being undertaken by his division in Canberra
Canberra, Australia
April 21, 2004

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Danny, can you give us an overview of some of the biotech projects that you and your team here are running at the moment?

OK Adam, we’ve got a number of different aspects of the work that’s going on here. A major component of it really is to provide molecular support to our transgenic cotton-breeding program based in Narrabri and that involves quite a bit of out time.

But the other component of it is to carry out some basic research into various aspects of the cotton plant that may in the longer term lead us to new products, which can be used as transgenics. This includes various things like looking at fibre development.

Obviously the output of a cotton plant is the fibre that you harvest but we have very little understanding about the basic molecular biology of hoe fibred develop on seeds so there are a number of projects going on at the moment using some of the latest technologies to try and get a better understanding of what genes are actually involved in the production of the cotton fibre with the long-term view hopefully of being able to manipulate fibre growth and development to improve quality characteristics of cotton varieties at the moment.

Can you tell me a little bit more about the technique you are using; the Micro array technique?

Yes, basically micro arrays are a new technology, part of a group of technologies that are grouped in terms of genomics, which is really to look at large numbers of genes rather than, in the past we’ve been able to isolate and characterise and manipulate single genes at a time but a lot of biological processes really don’t involve single genes, they involve large sets of genes that work together to produce a particular characteristic in a plant.

So some of these new technologies really just allow us to look at many different genes at a single time and one of those is using things called micro arrays, which are basically gene sequences which are spotted onto a glass slide, which allow us to probe the expression of different genes under different conditions.

For example we have here one of the micro arrays which we have on here about 10,000 different cotton gene sequences which we’re starting to use to look some of the genes which are involved in the early processes of cotton fibre initiation and this has led us to find a couple of candidate genes which we can now start to look at in more detail to see what their role id in fibre development and that’s part of a CRDC funded project as well as a projects funded by CSD.

There is some other work going on, on oils. I know it’s not your work but can you tell us a little bit about that?

Yes there’s another group here in our oil seed modification group in plant industry that are working on trying to improve the nutritional qualities of the oil that are produced by cotton. In general the oil that’s in the cottonseed is really a bi-product of cotton fibre production but using transgenic technologies now we can start to manipulate the types of oils that are found in the seed to try and improve the value of the cottonseed either for nutritional purposes or for even industrial purposes and so there are a number of projects which are moving ahead in the first field trial work is starting on some of those in the coming year of cottonseed with improved nutritional qualities, which hopefully will open up the market for cotton oil as an end product for cotton fibre production.

Can you talk a little bit about the work and your interaction with the office of the gene technology regulator?

Yes over the years I’ve had a very close involvement with the Australian regulatory bodies who control the release of transgenic organisms out into the environment initially with GMAC, the Genetic Manipulation Advisory Committee and them more recently with the OGTR which is the statutory body that’s replaced GMAC. We’ve been working very closely with them to try and define all the appropriate conditions for releasing transgenic cotton out into the field in the safest possible manner and this has also required us to do a lot of experimental work to demonstrate that once we put these genes out into the environment that we can contain them in field trials and not have them spread around the country. So, we’ve worked very closely with them to try and formulate the safest and the most open and transparent system of regulation of anywhere in the world for transgenic organisms.

Further Information:  Robert EveleighJohn Marshall, or Craig McDonald
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