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October, 2007
Source:
Plant Breeding News, Edition 183
An Electronic Newsletter of Applied Plant Breeding
Sponsored by FAO and Cornell University
From: L Michael J. Havey
USDA-ARS
University of Wisconsin
Madison, WI USA 53706
http://haveylab.hort.wisc.edu
Inbred line B8667 A&B
The United States
Department of Agriculture, Agricultural Research Service,
announces the release of onion inbred B8667 A&B, for the
production of red, long-day, well-storing hybrids. This
inbred line was developed by Dr. M.J. Havey and is round in
shape, dark red with color extending through the internal
rings of the bulb, firm, with good scale retention and
excellent storage quality when produced on muck soils.
B8667B is a F1MSMS2M3 from USDA Plant Introduction 262985
(‘Noord Holland Bloodred’) crossed with B5361B (a red inbred
developed by the late Dr. C.E. Peterson, but never
released). This inbred has a soluble-solids content of 13.4%
and is relatively pungent at 10.7 mM pyruvate per ml. The
cytoplasmic male-sterile A line is a BC7. Testcrosses of
B8667B to a series of male-sterile F1 lines
(MSU611-1A×MSU611B, MSU5718A×MSU8155B, B3350A×B2352B,
B1731A×MSU5785B, and B1750A×B1794B) produced only red bulbs
and yielded in the top one-third of commercial and
experimental hybrids evaluated over years at the Kincaid
Farm, Palmyra, WI.
Synthetic population‘Onion
Haploid (OH)-1’
The Agricultural Research
Service of the United States Department of Agriculture and
the University of Ljubljana
announce the release of the long-day onion synthetic
population ‘Onion Haploid (OH) -1’. The purpose of this
population is to serve as a responsive control for
extraction of gynogenic haploids of onion. Random plants
from the relatively responsive inbreds B2923B and B0223B
were evaluated for gynogenic haploid production as described
by Bohanec and Jakse (1999). Plants that produced relatively
high numbers of gynogenic haploids were self-pollinated
(Bohanec et al. 2003). Five S1 bulbs from each of 10
families (nine from B2923B and one from B0223B) were caged,
allowed to flower, and intercrossed using flies. Plants in
this synthetic produced on average 12 gynogenic haploids for
every 100 flowers plated. Bulbs of this synthetic population
are yellow with good storage quality. All plants in OH-1
should be homozygous recessive at the Ms locus, although
this has not been evaluated.
Bohanec, B., and M. Jakše. 1999. Variations in gynogenic
response among long-day onion (Allium cepa L.) accessions.
Plant Cell Rep. 18:737-742.
Bohanec, B., M. Jakše, and M.J. Havey. 2003. Genetic
analyses of gynogenetic haploid production in onion. J.
Amer. Soc. Hort. Sci. 128:571-574
Onion Synthetic Population
Sapporo-KI (SKI) -1 A&B
The Agricultural Research
Service of the United States Department of Agriculture
announces the release of the long-day onion synthetic
population ‘Sapporo-Ki (SKI) -1’ A&B. Sapporo-Ki is an
open-pollinated population grown on the Japanese island of
Hoikkaido and has relatively high frequencies of both S
cytoplasm and the dominant allele at the male-fertility
restoration locus (Ms) (Havey 1995). This synthetic combines
the earliness of Sapporo-Ki with maintenance of
cytoplasmic-male sterility. Random plants from Sapporo-Ki
were self-pollinated and testcrossed to male-sterile plants
of MSU611-1A×MSU611B or MSU5718A×MSU8155B. The S1 families
were evaluated for their cytoplasm using the molecular
markers as described by Havey (1993). Testcross families
from these N-cytoplasmic plants were scored for
male-fertility restoration (Gokce and Havey 2002). S1
families that were N-cytoplasmic and homozygous recessive at
Ms were selected. Five S1 bulbs from each of eight families
were caged, allowed to flower, and intercrossed using flies,
followed by three generations of seed increases. The
cytoplasmic male-sterile A line is a BC5. Bulbs of this
synthetic population are yellow, very early maturing in
Wisconsin, and have good storage ability.
Havey, M.J. 1993. A putative donor of S-cytoplasm and its
distribution among open-pollinated populations of onion.
Theor. Appl. Genet. 86:128-134.
Havey, M.J. 1995. Cytoplasmic determinations using the
polymerase chain reaction to aid in the extraction of
maintainer lines from open-pollinated populations of onion.
Theor. Appl. Genet. 90:263-268.
Gokce, A.F., and M.J. Havey. 2002. Linkage equilibrium among
tightly linked RFLPs and the Ms locus in open-pollinated
onion populations. J. Amer. Soc. Hort. Sci. 127:944-946.
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