News section
Natural occurrence of chilli veinal mottle virus on Capsicum chinense in China

A ProMED-mail post
ProMED-mail is a program of the International Society for Infectious Diseases

February 23, 2006
From: ProMED-mail<promed@promedmail.org>
Source: American Phytopathological Society, Plant Disease Notes, March 2006 [edited] <http://www.apsnet.org/pd/searchnotes/2006/PD-90-0377C.asp >

Natural occurrence of chilli veinal mottle virus on Capsicum chinense in China
J. Wang, Z. Liu, S. Niu, M. Peng, and D. Wang, State Key Laboratory for Tropical Crop Biotechnology, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science, Haikou, Hainan, China 571101; and Z. Weng and Z. Xiong, Division of Plant Pathology and Microbiology, Department of Plant Sciences, University of Arizona, Tucson, AZ 85749. Plant Dis. 90:377, 2006; published on-line as DOI: 10.1094/PD-90-0377C. Accepted for publication 30 Dec 2005.

An outbreak of a viral disease on chili pepper (_Capsicum chinense_ Jacp. cv. Yellow Lantern) occurred in Hainan Province, China during 2003 and 2004. The disease was prevalent in 5 chili-producing counties surveyed. Leaves of infected plants initially displayed symptoms of dark green banding along veins and later became distorted with striking mosaic. Infected plants had reduced flower numbers and fruit set, resulting in a significant yield loss. The
causative virus was characterized and identified as Chilli veinal mottle virus (ChiVMV) (3). An isolate of the virus was obtained via 3 single lesion passages through _Chenopodium amaranticolor_ and was shown to reproduce the same symptoms on inoculated _C. chinense_ cv. Yellow Lantern. Negative staining of crude extracts of the infected tissue and subsequent electron microscopy revealed flexuous rods of 12-13 x 750 nm, typical of a potyvirus. Pinwheel-like inclusion bodies were abundant in thin sections of infected leaves. Purified virus preparations contained one major protein of 32.8 kDa and one minor protein of 28 kDa when fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Both of these protein bands were excised and subsequently analyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Multiple peptide fragments from both proteins were identified as arising from ChiVMV capsid protein (CP) (1,2). Therefore, the 32.8-kDa protein is the full-length ChiVMV CP and the 28-kDa protein is presumably a degradation product of the CP. The combined biological and molecular data provided strong evidence that the viral disease on _C. chinense_ was caused by ChiVMV.

To our knowledge, this is the first report of ChiVMV infection on _C. chinense_ in China and the first report of _C. amaranticolor_ as an experimental host for ChiVMV.

References: (1) P. Chiemsombat et al. Arch. Virol. 143:1855, 1998. (2). J. Joseph and H. S. Savithri. Arch. Virol. 144:1679, 1999. (3) P. Siriwong et al. Plant Pathol. 44:718, 1995.

--
ProMED-mail
<promed@promedmail.org>

[The aphid-transmitted potyvirus chilli veinal mottle virus (ChiVMV) is reported here for the first time in chili pepper (_Capsicum chinense_) in Hainan Province, China from samples collected in 2003 and 2004. ChiVMV is one of the most predominant viruses of peppers in Asia. Surveys in 16 Asian countries have shown that 30 percent of pepper crops are affected by this disease. Flower drop is normally severe following infection with this virus and therefore crop loss can be heavy. Growing transplants in insect-proof facilities, avoiding weeds and other solanaceous crops (e.g. tomato), planting early to avoid aphid flights, and using mineral oil sprays are methods used in attempts to manage the disease.

_Capsicum chinense_, the crop plant infected, remains the least understood of the domesticated pepper taxa with respect to center of origin and its probable progenitor. Fruit shape can vary from long and slender to short and obtuse. Fruit can be extremely pungent and aromatic, with persistent pungency when eaten. The best-known cultivars are the very hot Habanero peppers, such as Scotch Bonnet. The see also section lists past postings on viruses of pepper. This post is the first on ChiVMV.

Map:
Hainan Province, China.
<http://www.paulnoll.com/China/Provinces/Province-Hainan.html >
Pictures:
Diseased peppers.
<http://www.avrdc.org/news/04chivmv.html >
_Capsicum chinense_.
<http://www.chilepepperinstitute.org/Images/habanero2.JPG >

Links:
<http://www.avrdc.org/pdf/pepper/ChiVMV.pdf >
<http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/57010016.htm >
<http://image.fs.uidaho.edu/vide/descr209.htm>
<http://www.g6csy.net/chile/var-chi.html >
- Mod.JAD]

[see also in the
archive:
2005
----
Bell pepper leaf curl virus - Pakistan (Lahore) 20051022.3084
2004
----
Tomato leaf curl virus, chilli pepper - Pakistan 20040509.1255
Pepper golden mosaic virus, tomato - Mexico 20040214.0490
2003
----
Tobacco mosaic virus, pepper - USA (New York) 20030212.0376
2002
----
Broad bean wilt virus-1, pepper - Spain 20020707.4681
2001
----
Pepper mild mottle virus, pepper - USA (Florida) 20010720.1405]
 

ISID/ProMED-mail post news item

Other releases from this source

14,997

Back to main news page

The news release or news item on this page is copyright © 2005 by the organization where it originated.
The content of the SeedQuest website is copyright © 1992-2005 by
SeedQuest - All rights reserved
Fair Use Notice