Date of publication: February 21,
2006
Source:
http://gmoinfo.jrc.it/gmp_browse_geninf.asp
Notification number: B/DE/05/171
Member State:Germany
Date of Acknowledgement:10/11/2005
Title of the Project:
Development of a standardised method to minimise out crossing
of transgenic oilseed rape in field experiments
Proposed period of release From:01/04/2006
To:31/10/2007
Name of the Institute(s) or Company(ies): Verein zur
Förderung Innovativer und nachhaltiger AgroBiotechnologie MV -
FINAB e.V.;
3. Is the same GMPt release planned elsewhere in the
Community?
No
4 - Has the same GMPt been notified elsewhere by the same
notifier?
No
Genetically
modified plant
1. Complete name of the
recipient or parental plant(s)
|
Common Name
|
Family Name
|
Genus |
Species
|
Subspecies
|
Cultivar/breeding line
|
| spring
oilseed rape |
brassicaceae |
brassica |
brassica napus |
napus
(syn. oleifera) |
Drakkar, Lisora |
2. Description of the traits and characteristics which have
been introduced or modified, including marker genes and previous
modifications:
Genes for either stilbensynthase (VST1), and/or suppression
cassettes (sense – spacer – antisense) for the sinapic acid
glycolsyl transferase (SGT) and/or sinapoylglucose cholin
sinapoyl transferase (SCT) genes were introduced into spring
oilseed rape in order to either synthesise the health-improving
compound resveratrol and/or to reduce the phenylic compound
sinapine. Either the neomycin phosphotransferase II gene (nptII)
or the phosphinothricine acetyltransferase gene (bar) each under
control of the 35S promoter have been used as selectable marker
gene.
Genetic
modification
3. Type of genetic
modification:
Insertion;
4. In case of insertion of genetic material, give the source
and intended function of each constituent fragment of the region
to be inserted:
The genes of interest are VST I (stilbensynthase) taken from
Vitis vinifera, and suppression cassettes for the SGT (sinapic
acid glycolsy transferase) and SCT (sinapoylglucose cholin
sinapoyl transferase) genes, each taken from Brassica napus. The
genes are used alone and in combination.
These genes of interest are regulated by the napin promoter from
Brassica napus.
The terminators used are:
- an endogeneous terminator of Vitis vinifera,
- the nos terminator from A. tumefaciens,
- the 35S terminator from Cauliflower mosaic virus.
As selectable marker genes the neomycin phosphotransferase gene
(npt II) or the, phosphinothricine acetyltransferase gene (bar)
from Streptomyces hygroscopicus, each flanked by the 35S
promoter and 35S terminator from cauliflower mosaic virus, have
been used.
6. Brief description of the method used for the genetic
modification:
An Agrobacterium-mediated transformation and plant
regeneration system slightly modified according to DE BLOCK et
al. (1989) was used for the transformation of spring oilseed
rape cultivars Drakkar and Lisora.
7. If the recipient or parental plant is a forest tree
species, describe ways and extent of dissemination and specific
factors affecting dissemination:
Not applicable.
Experimental
Release
1. Purpose of the release:
Evaluation of trait performance under field conditions and
assessment of various methods to minimise out crossing of
transgenic oilseed rape in release trials.
2. Geographical location of the site:
Sanitz (Mecklenburg-Vorpommern)
3. Size of the site (m2):
Total area of genetically modified oilseed rape will not
exceed 480 m2.
4. Relevant data regarding previous releases carried out with
the same GM-plant, if any, specifically related to the potential
environmental and human health impacts from the release:
Not applicable.
Environmental
Impact and Risk Management
Summary of the potential
environmental impact from the release of the GMPts:
There is no scientific reason to assume that the
modifications by biosynthesis of resveratrol glucoside and/or
reduction of sinapine content may lead to changes in
reproduction, dispersion, persistence or invasiveness of these
plants compared to conventional oilseed rape. On basis of
current experience the transgenic plants do not differ from
non-transgenic plants in growth, size, phenology and seed
formation. There is no known selection pressure in favour for or
against the presence of resveratrol glucoside or low sinapine or
a combination of both characters in seeds. Due to current
experience no effect of the resveratrol glucoside in the seeds
on pest or beneficial organisms or on health and environment is
expected.
Brief description of any measures taken for the management of
risks:
In order to minimise cross-pollination, transgenic field
plots are surrounded by either fertile conventional and/or
male-sterile oilseed rape. The distance of the nearest
cultivation of oilseed rape plants will be at least 1,5 km.
Sowing and harvesting machinery will be cleaned on site to
prevent the dispersal of GM seed. Harvested seed material will
be transported from the site in closed and labelled containers
to the laboratories for analyses. Remaining seed will be
inactivated. Vegetative plant material will be chopped and
worked into the soil. The area will be controlled for volunteers
for a period of 4 consecutive years. During the release period
the Field Manager and trained personnel will monitor the trial
site at defined intervals.
Summary of foreseen field trial studies focused to gain new
data on environmental and human health impact from the release:
The field trials are designed to investigate the pollen flow
influenced by different kinds of protective borders and to find
the optimal method to minimise transgenic pollen flow.
Final report
-
European
Commission administrative information
Consent given by the Competent
Authority: Not Known |
