Date of publication: February 14,
2006
Source:
http://gmoinfo.jrc.it/gmp_browse_geninf.asp
Notification number:
B/ES/06/26
Member State:Spain
Date of Acknowledgement:28/12/2005
Title of the Project:
Testing of genetically modified 59122xNK603
(DAS-59122-7xMON-ØØ6Ø3-6) maize.
Proposed period of release From:01/04/2006
To:31/12/2009
Name of the Institute(s) or Company(ies): PIONEER
HI-BRED SPAIN S.L.;
3. Is the same GMPt release planned elsewhere in the
Community?
No
4 - Has the same GMPt been notified elsewhere by the same
notifier?
No
Genetically
modified plant
1. Complete name of the
recipient or parental plant(s)
|
Common Name
|
Family Name
|
Genus |
Species
|
Subspecies
|
Cultivar/breeding line
|
| maize
|
poaceae |
zea
|
zea
mays |
mays
|
Experimental varieties |
2. Description of the traits and characteristics which have
been introduced or modified, including marker genes and previous
modifications:
The genetically modified 59122xNK603 maize varieties that
will be tested in these field trials are resistant to certain
Coleopteran insects, such as the Western corn rootworm
(Diabrotica virgifera virgifera) and tolerant to
glufosinate-ammonium and glyphosate herbicides. This maize was
derived through traditional breeding methods between progeny of
genetically modified 59122 maize, which is resistant to certain
coleopteran insects and tolerant to glufosinate herbicide, and
NK603 maize (from Monsanto), which is tolerant to glyphosate
herbicide.
Genetic
modification
3. Type of genetic
modification:
Insertion;
4. In case of insertion of genetic material, give the source
and intended function of each constituent fragment of the region
to be inserted:
The genes introduced in 59122xNK603 maize are the cry34Ab1
and cry35Ab1 genes from Bacillus thuringiensis strain PS149B1,
providing resistance to certain coleopteran insects and the pat
gene from Streptomyces viridochromogenes, introduced as a
selectable marker, providing tolerance to glufosinate herbicide,
like in 59122 maize, and the cp4epsps gene from Agrobacterium
sp. strain CP4 that confers tolerance to glyphosate herbicide,
like in NK603 maize, along with their regulatory components
necessary for expression in maize plant:
- the cry34Ab1 gene, the UBIZM1(2) promoter, the PINII
terminator.
- the cry35Ab1 gene, the Peroxidase promoter, the PINII
terminator.
- the pat gene, the CaMV35S promoter, the CaMV35S terminator.
- the cp4epsps gene, the P-ract1/ract1 intron + ctp2, the NOS 3’
terminator.
- the cp4epsps gene, the CaMV e35S promoter + Zmhsp70 + ctp2,
the NOS 3’ terminator.
6. Brief description of the method used for the genetic
modification:
No genetic modification was made on the 59122xNK603 maize
varieties directly: they were derived through traditional
breeding methods between progeny of the genetically modified
59122 maize and NK603 maize.
59122 maize was genetically modified using Agrobacterium
transformation method. NK603 maize was genetically modified by
Monsanto using the particle acceleration method.
7. If the recipient or parental plant is a forest tree
species, describe ways and extent of dissemination and specific
factors affecting dissemination:
Not applicable.
Experimental
Release
1. Purpose of the release:
Several types of trials are planned, to collect various
59122xNK603 maize tissue samples for protein expression
analysis, to evaluate the impact of the cultivation of
transgenic maize varieties on key non-target arthropod
populations, to control maize volunteers with different
herbicides, to evaluate the glyphosate effects on transgenic
maize varieties.
2. Geographical location of the site:
The release is planned at the following locations in Spain in
2006:
- Aragón: Zuera (Zaragoza), Gurrea de Gállego (Huesca).
- Castilla-La Mancha: Tarazona de la Mancha (Albacete), Alpera
(Albacete), Membrilla (Ciudad Real).
- Cataluña: Alpicat (Lleida), Alguaire (Lleida).
- Madrid: San Martín de la Vega (Madrid).
- Navarra: Cortes (Navarra).
3. Size of the site (m2):
In 2006, each site could have up to 500 m² sown with the
genetically modified maize subject of this notification
(59122xNK603 maize), the total trial surface (all varieties and
borders included) being larger at each site. In 2006, the site
in Alguaire (Cataluña) could have up to 8000m² sown with
59122xNK603 maize, as the trial to evaluate the impact on key
non-target arthropod populations will be carried out in
collaboration with the University of Lérida. The total trial
surface (all varieties and borders included) will be larger.
In the following years, there could have up to ten sites and up
to 10000 m² of 59122xNK603 maize sown per site, the total trial
surface at each site (all varieties and borders included) being
larger.
4. Relevant data regarding previous releases carried out with
the same GM-plant, if any, specifically related to the potential
environmental and human health impacts from the release:
Field trials have already been conducted at several locations
across maize growing regions in North America, South America and
Europe and no environmental problems were reported for these
trials. The transgenic plants were indistinguishable from the
non-genetically modified maize plants except for showing
resistance to certain Coleopteran insects such as the Western
corn rootworm and showing tolerance when glufosinate and
glyphosate herbicides have been applied, traits due to the
genetic modifications.
Environmental
Impact and Risk Management
Summary of the potential
environmental impact from the release of the GMPts:
No risks to human and animal health or the environment from
the deliberate release of genetically modified maize resistant
to certain Coleopteran insects and tolerant to glufosinate and
glyphosate herbicides are expected, as per information contained
in the environmental risk assessment included in the
notification.
Brief description of any measures taken for the management of
risks:
The shedding of pollen from the genetically modified plants
will be controlled by maintaining a 200-meter isolation distance
with any other non-experimental maize crop. In addition, the
trial site will be surrounded by four border rows of
conventional maize of a similar relative maturity that will be
also destroyed at the end of the release.
Seed dispersal of individual kernels does not generally occur.
They are fixed on a cob and enclosed in many husks that protect
the seeds from outside contact. When a few seeds need to be
collected for analysis; this will be done by sampling the whole
ear and unused seeds will be destroyed.
In case of any emergency, the trial could be stopped by
application of a non-selective herbicide, other than glufosinate
and glyphosate, or by mechanical destruction, and incorporation
into the soil.
At the end of the release, all remaining plant matter that has
not been harvested for analyses will be destroyed by chopping
and incorporating into the soil. No plant or plant product
coming from the trials will enter the food or feed chains.
Volunteer maize will be monitored during the following year. An
herbicide treatment (other than glufosinate and glyphosate) will
be used in order to ensure destruction if needed.
There will be no commercial maize grown on the same field the
following year.
Summary of foreseen field trial studies focused to gain new
data on environmental and human health impact from the release:
Not applicable to this release.
Final report
-
European
Commission administrative information
Consent given by the Competent Authority: Not Known |
