Mention "biotechnology" to most Australians and chances are
they will interpret the word as meaning genetic modification. Of
food and animals. In some scientific conspiracy to manipulate
the natural order.
That’s a pity, because the great bulk of biotechnology does
not involve genetic modification.
Take "doubled haploid" technology, for instance. The name
itself suggests something a bit complicated and the process
actually is a bit that way.
Yet it is being used by the breeding teams responsible for
maintaining the quality and market relevance of Australian
wheats and barleys to slash as many as four years from the time
traditionally taken to turn out a new, improved variety.
The first example of what doubled haploid technology can do
for the Australian grains industry came a few weeks back, with
the commercial release of the new Australian Prime Hard wheat
variety EGA Hume.
Making the official release, David Hamilton, director of the
Queensland Department of Primary Industries Farming Systems
Institute, said the EGA Hume was a significant milestone for the
Australian grain industry, being the nation’s first wheat
variety produced using doubled haploid technology.
QDPI has had doubled haploid capability since the early
1990s, recently improved with the construction of a dedicated,
solar, passive, low-input glasshouse. There are also
laboratories at the University of Sydney, South Australia’s
Research and Development Institute and Western Australia’s
Department of Agriculture using the same technology.
All are involved in the national crop breeding programs
supported and coordinated by Australian growers and the Federal
Government through the Grains Research &
Development Corporation.
Ross Gilmour, manager of the GRDC’s winter cereal improvement
program, says the advantages of doubled haploid technology come
after the initial cross of two chosen parent lines.
"In the generations after a cross is made, lines segregate
for the characters by which the two parents differ," he says.
"In traditional wheat breeding, at least four generations of
self-pollination are necessary to fix the genes so the lines
breed true to type in future generations and become suitable for
commercial production.
"The doubled haploid technique circumvents the need for the
multiple generations of self-pollination to fix the lines.
"In wheat – the technique is a bit different for barley – the
doubled haploid process involves making a cross between two
parent lines, growing the seed that results from that cross and
then crossing to those plants with maize pollen.
"Crossing with maize and subsequently applying a chemical
called colchicine has the effect of duplicating the chromosomes
exactly, thereby fixing the genes they carry.
"The lines are fixed in the space of one generation, rather
than the four or five generations required using a standard
(pedigree) breeding procedure.
" The time required to develop a new variety is reduced by up
to four years as a result."
Dr Gilmour says strategic use of the doubled haploid
technique, in combination with molecular markers for desirable
traits presents even greater opportunities to accelerate the
rate of genetic progress through plant breeding.
The doubled haploid technique requires special infrastructure
– growth rooms and temperature and humidity controlled
glasshouses – and is labour intensive.
"Producing a doubled haploid wheat plant costs about $15 and
several hundreds to several thousands of them must be produced
in a breeding program to make it an effective breeding
technique," he says.