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A combination of germplasm, genetics, genomics, and stress physiology identify tomatoes with tolerance to
drought and high temperatures

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Tainan, Taiwan
May 29, 2009

Source: AVRDC - The World Vegetable Center - Newsletter

Global food security depends on genetic diversity. To develop vegetable crops capable of thriving in the extremes of a changing climate, plant breeders must evaluate hundreds of crop relatives in search of genes linked to important agronomic traits such as drought tolerance and disease resistance. Once identified, these genes can be built into commercial varieties through conventional breeding or genetic engineering.

 

Performance of AVRDC-developed heat tolerant tomato varieties in Tanzania. Drought pool screening method for comparative assessment of drought tolerance among plants under the same soil moisture conditions.

You could say our breeders are spoiled for choice: AVRDC maintains the world’s largest public vegetable genebank with over 56,000 accessions from 150 countries, including over 44,000 accessions of globally important vegetables and close to 12,000 accessions of indigenous vegetables. Yet even with a wealth
of germplasm at hand, the right gene or combination of genes may remain elusive. Although molecular characterization and genetic diversity analysis of germplasm collections greatly enhances the efficiency of breeding programs in identifying key genes, breeding for tolerance to environmental (abiotic) stress continues to challenge plant breeders. Refining methods to evaluate phenotype—a plant’s observable physical characteristics, which are a product of its genes and its response to the environment it is growing in—can reveal a great deal about its defense mechanisms.

Solanum pennellii accession showing drought tolerance.

 

Pollen viability test for heat tolerance in tomato indicating viable pollen.

The drought pool screening method used by AVRDC researcher Dr. Rachael Symonds has been useful in assessing the comparative drought tolerance of different lines or germplasm accessions under the same soil moisture conditions. “The drought pool is a simple and practical method that allows a large number of genotypes to be screened quickly for drought tolerance,” says Dr. Symonds. “Plants have to compete against each other for the same soil water, so genotypes with a competitive advantage are easily identified.” She also has developed protocols to monitor the transpiration response of test plants to a progressive water deficit. By applying these screening protocols, drought tolerance was identified in Solanum pimpinellifolium and S. pennellii, wild relatives of cultivated tomatoes.

A prolific tomato line may stop producing fruit when temperatures start to climb. In high heat, tomatoes
release less pollen, and less viable pollen, which decreases fruit set and yield. Selecting tomatoes for pollen
viability under heat stress was found to be another valuable screening method for heat tolerance.

Vegetable cultivars with tolerance to heat and drought would help improve the livelihoods of resource poor farmers in developing countries. AVRDC – The World Vegetable Center together with its partners the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), India; Leibniz University of Hannover, Germany; Central Queensland University (CQU), Australia; University of Dar-es-Salaam (UDES), Tanzania; Mikocheni Agricultural Research Institute (MARI), Tanzania; and Academia Sinica, Taiwan is
addressing the problem of drought and heat stress in tomato through a project funded by BMZ/GTZ to improve productivity and enhance yield stability of tomato in the tropics.

The heat tolerance trials conducted at HQ and at UDES, Tanzania demonstrated that heat tolerant varieties developed at AVRDC-HQ are useful sources for Africa as well. In a farmer participatory evaluation trial conducted by our partner, the University of Dar-es-Salaam, Tanzania, the AVRDC-developed heat tolerant tomato varieties were highly preferred by farmers.

by Andreas Ebert and Kadirvel Palchamy

 

 

 

 

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