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May, 2007
Source:
CropBiotech Update
DNA markers have helped a lot in speeding up several steps in
the plant breeding process. Among its many applications include
use in marker assisted selection for superior genotypes, and for
checking for genotype uniformity.
DNA marker analysis can be automated to meet both the
high-throughput and low cost requirement of many breeding
programs, says researchers in Sweden and Denmark. The group
presented the fully automated polymerase chain reaction system
used in Svalof Weibull AB
(SW) for evaluating barley and canola lines.
The system was presented to be capable of analyzing up to 2200
samples per day at a cost of 0,24 € per analysis for marker
assisted selection and quality control of genetically modified
organisms.
The complete paper with the detailed description of the SW
system was published by the journal Plant Breeding and available
for subscribers at
http://www.blackwell-synergy.com/doi/abs/10.1111/j.1439-0523.2007.01306.x.
ABSTRACT
Automation of DNA marker
analysis for molecular breeding in crops: practical experience
of a plant breeding company
C. Dayteg,
Svalöf Weibull AB, SW Laboratory, SE-26181 Svalöv, Sweden
S. Tuvesson,
Svalöf Weibull AB, SW Laboratory, SE-26181 Svalöv, Sweden
A. Merker, Department of Crop Science, Swedish University of
Agricultural Sciences, Box 44, SE-23053 Alnarp, Sweden
A. Jahoor, Plant and Soil Science Laboratory, Department of
Agricultural Sciences, The Royal Veterinary and Agriculture
University, Thorvaldssensvej 40, DK-1871 Fredriksberg, C,
Copenhagen, Denmark and
A. Kolodinska-Brantestam, Department of Crop Science, Swedish
University of Agricultural Sciences, Box 44, SE-23053 Alnarp,
Sweden
Plant Breeding (OnlineEarly Articles).
doi:10.1111/j.1439-0523.2007.01306.x
In modern plant breeding, DNA marker analyses are of increasing
importance and, as the methods become more widely adopted, the
capacity for high-throughput analyses at low cost is crucial for
its practical use. Automation of the analysis processes is a way
to meet these requirements. In order to achieve this, while
keeping adequate flexibility in the analysis processes, Svalöf
Weibull AB (SW) has developed a fully automated polymerase chain
reaction system. It has been evaluated on barley and canola
lines and is capable of analysing up to 2200 samples per day at
a cost of 0,24 € per analysis for marker-assisted selection and
quality control of genetically modified organisms. A detailed
description of this system is given, and improvements to the
throughput and applications are discussed. |
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