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June 15, 2007
Source:
CropBiotech Update
RNA interference (RNAi) was used
to obtain a common bean line resistant to bean golden mosaic
virus (BGMV), the virus responsible for golden mosaic disease in
the crop. The research, conducted in Brazil, reports that 93% of
the plants from the transgenic resistant line were free of
symptoms upon high pressure inoculation.
BGMV is a major constraint in bean production that causes yield
losses between 40 to 100%. The virus is transmitted by the
whitefly Bemisia tabaci. The RNAi approach uses an RNA
interference construct to silence the sequence region of the AC1
viral gene, producing resistant common bean. Compared to the
non-transgenic control with 100% golden mosaic incidence after
38 days of inoculation, the transgenic line has only 7.8%
disease incidence.
For details the complete paper published in
Molecular
Plant-Microbe Interactions can be accessed by subscribers at
http://www.apsnet.org/mpmi/SubscriberContent/2007/MPMI-20-6-0717.pdf.
RNAi-Mediated Resistance
to Bean golden mosaic virus in Genetically Engineered Common
Bean (Phaseolus vulgaris).
K. Bonfim, J. C. Faria, E. O. P. L. Nogueira, É. A. Mendes,
and F. J. L. Aragão. Pages 717-726.
Molecular Plant-Microbe Interactions
June 2007
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