January, 2007
Source:
Plant
Biotechnology Journal,
Blackwell Synergy
'GM-gene-deletor': fused
loxP-FRT recognition sequences dramatically improve the
efficiency of FLP or CRE recombinase on transgene excision from
pollen and seed of tobacco plants
Keming Luo, Hui Duan, Degang Zhao, Xuelian Zheng, Wei Deng,
Yongqin Chen, C. Neal Stewart Jr, Richard McAvoy, Xiangning
Jiang, Yanhong Wu, Aigong He, Yan Pei, Yi Li
Plant
Biotechnology Journal (OnlineEarly Articles).
doi:10.1111/j.1467-7652.2006.00237.x
http://www.blackwell-synergy.com/doi/abs/10.1111/j.1467-7652.2006.00237.x
SUMMARY
Pollen- and seed-mediated
transgene flow is a concern in plant biotechnology. We report
here a highly efficient 'genetically modified (GM)-gene-deletor'
system to remove all functional transgenes from pollen, seed or
both. With the three pollen- and/or seed-specific gene promoters
tested, the phage CRE/loxP or yeast FLP/FRT system alone was
inefficient in excising transgenes from tobacco pollen and/or
seed, with no transgenic event having 100% efficiency. When
loxP-FRT fusion sequences were used as recognition sites,
simultaneous expression of both FLP and CRE reduced the average
excision efficiency, but the expression of FLP or CRE alone
increased the average excision efficiency, with many transgenic
events being 100% efficient based on more than 25 000 T1 progeny
examined per event. The 'GM-gene-deletor' reported here may be
used to produce 'non-transgenic' pollen and/or seed from
transgenic plants and to provide a bioconfinement tool for
transgenic crops and perennials, with special applicability
towards vegetatively propagated plants and trees.
Article:
http://www.blackwell-synergy.com/doi/abs/10.1111/j.1467-7652.2006.00237.x |