Date of publication: August 9,
2006
Source:
http://gmoinfo.jrc.it/gmp_browse_geninf.asp
Notification number:
B/DE/06/178
Member State: Germany
Date of Acknowledgement: 03/08/2006
Title of the Project: Increase of grain protein
content in winter wheat
Proposed period of release From:01/10/2006
To:31/08/2008
Name of the Institute(s) or Company(ies):
Leibniz-Institut of Plant Genetics and Crop Plant Research (IPK);
3. Is the same GMPt release planned elsewhere in the
Community?
No
4 - Has the same GMPt been notified elsewhere by the same
notifier?
No
Genetically
modified plant
1. Complete name of the
recipient or parental plant(s)
|
Common Name
|
Family Name
|
Genus |
Species
|
Subspecies
|
Cultivar/breeding line
|
| wheat
|
poaceae |
triticum |
triticum aestivum |
|
Certo
|
2. Description of the traits and characteristics which have
been introduced or modified, including marker genes and previous
modifications:
Triticum aestivum (cv. Certo) has been ballistically
transformed. The transgenic winter wheat plants (termed basis
lines) contain: a barley-derived sucrose transporter (HvSUT1)
under control of the hordein B1 promoter of barley (line HOSUT),
a Vicia faba-derived amino acid permease under control of the
1Ax1 promoter of wheat (line XAP) and under control of the
HvSUT1 promotor (line SUTAP). The lines contain the marker genes
phosphinotricin acyl transferase (bar)conferring resistance
against glufosinate-ammonium (HOSUT, XAP, SUTAP) and
beta-lactamase conferring resistance against ampicillin (XAP,
SUTAP). All lines could putatively contain the cat-gene
conferring resistance against chloramphenicol but were not
tested for. At green house conditions, expression of the
transporter genes in winter wheat plants increases the grain
protein content up to 30%. In addition, lines XAP and SUTAP show
an early flowering phenotype. These transgenic basis lines were
crossed into seven non transgenically modified winter wheat
lines from a breeding company. After crossing, 816 individually
different homozygous transgenic lines were developed containing
one of the described target genes (wheat breeding nursery).
Influence of transgene expression on grain composition and
flowering time of the breeding nursery lines was not analysed up
to now.
Genetic
modification
3. Type of genetic
modification:
Insertion;
4. In case of insertion of genetic material, give the source
and intended function of each constituent fragment of the region
to be inserted:
1.
Name: 1Ax1-Promotor
Function: regulatory subunit of seed storage protein Ax2
Source: Triticum aestivum var. Cheyenne
Length of nucleotide/
amino acid sequence: 2376 bp (Pos. 1…2376)
Accession/Reference: M22208.2; GI:14043051
2.
Name: Hordein-Promotor
Function: regulatory subunit of seed storage protein hordein B1
Source: Hordeum vulgare var. Carlsberg II
Length of nucleotide/
amino acid sequence: 550 bp (Pos. 1...550)
Accession/Reference: X87232.1; GI:809030
3.
Name: HvSUT1-Promotor
Function: regulatory subunit of sucrose transporters 1 (sut1)
Source: Hordeum vulgare subsp. vulgare
Length of nucleotide/
amino acid sequence: 1445 bp (Pos. 1...1445)
Accession/Reference: Wang Q. (2003)
4.
Name: VfAAP1
Function: Amino acid permease 1 (aap1)
Source: Vicia faba var. minor
Length of nucleotide/
amino acid sequence: 1427 bp (Pos. 39...1466)
Accession/Reference: AJ318809; GI:15216025
5.
Name: VfAAP1
Function: Amino acid permease 1 (AAP1)
Source: Vicia faba var. minor
Length of nucleotide/
amino acid sequence: 475 AS
Accession/Reference: CAC51423; GI:15216026
6.
Name: HvSUT1
Function: Sucrose transporter 1 (sut1)
Source: Hordeum vulgare subsp. vulgare
Length of nucleotide/
amino acid sequence: 2044 bp (Pos. 1...2044)
Accession/Reference: AJ272309.1; GI:7024414
7.
Name: HvSUT1
Function: Sucrose transporter 1 (SUT1)
Source: Hordeum vulgare subsp. vulgare
Length of nucleotide/
amino acid sequence: 523 AS
Accession/Reference: CAB75882.1; GI:7024415
8.
Name: Hordein terminator
Function: Terminator of seed storage protein hordein B1
Source: Hordeum vulgare var. Carlsberg II
Length of nucleotide/
amino acid sequence: 1660 bp (Pos. 1...1660)
Accession/Reference: Brandt A. (1985)
9.
Name: OCS terminator
Function: Terminator of Octopin synthetase
Source: Agrobacterium tumefaciens
Length of nucleotide/
amino acid sequence: 727 bp (Pos. 1...727)
Accession/Reference: AF242881.1; GI:8572673
6. Brief description of the method used for the genetic
modification:
not applicable
7. If the recipient or parental plant is a forest tree
species, describe ways and extent of dissemination and specific
factors affecting dissemination:
not applicable
Experimental
Release
1. Purpose of the release:
Expression of transport proteins aims at increasing of sink
strength of the developing winter wheat grains. The transgenic
lines show at green house conditions an up to 30% increase of
the grain protein content and early flowering phenotype. The two
new traits are of high agronomical relevance. Verification of
the results by growing of the transgenic plants at outdoor
environmental conditions and combination with traits being
additionally relevant for breeders (as represented by the
conventional winter wheat lines from the breeding company) may
result in new agriculturally useful breeding lines.
2. Geographical location of the site:
Gatersleben, Saxonia-Anhalt, Germany
3. Size of the site (m2):
Transgenic base lines: 150 m2
Transgenic breeding nursery: 1,050 m2 (35 m x 30 m)
4. Relevant data regarding previous releases carried out with
the same GM-plant, if any, specifically related to the potential
environmental and human health impacts from the release:
not applicable
Environmental
Impact and Risk Management
Summary of the potential
environmental impact from the release of the GMPts:
The transgenic plants confer resistance against the herbicide
BASTA. However, every herbicide different from BASTA can be used
to destroy the transgenic winter wheat plants if necessary. No
significant environmental benefit can be derived from the
transgenes integrated.
Brief description of any measures taken for the management of
risks:
The release area will be surrounded by Phacelia at a width of
5 m to isolate the site; at least 20 m of distance will be
maintained to any agriculturally used area, pollen dispersal is
prevented by using winter wheat as transgenic crop as wheat is
largely self-fertilising; seed dispersal is prevented by using
of bird-scaring equipment and field spanning nets during grain
ripening; the release area is monitored during the following
year.
Summary of foreseen field trial studies focused to gain new
data on environmental and human health impact from the release:
not applicable
Final report
-
European
Commission administrative information
Consent given by the Competent
Authority: Not Known |
