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Deliberate release into the E.U. environment of GMOs for any other purposes than placing on the market:
Notification for the field testing of spring oilseed rape (Brassica napus) genetically modified for improved oil composition in the seed
Date of publication: November 29, 2006
Source: http://gmoinfo.jrc.it/gmp_browse_geninf.asp

Notification number: B/SE/06/11080

Member State: Sweden

Date of Acknowledgement: 16/11/2006 00:00:00

Title of the Project
Notification for the field testing of spring oilseed rape (Brassica napus) genetically modified for improved oil composition in the seed

Proposed period of release: 01/01/2007 00:00:00 to 31/12/2011 00:00:00

Name of the Institute(s) or Company(ies)
Plant Science Sweden AB, Herman Ehles väg 2-4
SE-268 31 Svalöv
Sweden;

3. Is the same GMPt release planned elsewhere in the Community?
Yes:

Has the same GMPt been notified elsewhere by the same notifier?
Yes

If yes, notification number(s):

Other notifications
Chile

Genetically modified plant

Complete name of the recipient or parental plant(s)
 
Common Name Family Name Genus Species Subspecies Cultivar/breeding line
oilseed rape brassicaceae brassica brassica napus napus (syn. oleifera) Westar

2. Description of the traits and characteristics which have been introduced or modified, including marker genes and previous modifications:
- Genes that are involved in the regulation of the biosynthesis of storage compounds in seeds have been introduced, in order to improve the oil quality in the seeds.

- nptII gene (neomycin phosphotransferase II) as selectable marker gene to identify transgenic cells in tissue culture


Genetic modification

3. Type of genetic modification:
Insertion;

In case of insertion of genetic material, give the source and intended function of each constituent fragment of the region to be inserted:
- napin promoter from Brassica napus
- D6 desaturase gene from Phytium irregulare
- D5 desaturase gene from Thraustochytrium spp.
- D6 elongase gene from Physcomitrella patens
- D12 desaturase gene from Calendula officinalis
- ocs (octopin synthase) terminator from Agrobacterium tumefaciens
- recombination sites mr-attB1 and mr-attB2 used for construction of the vector
- Promoter and terminator from nopaline synthase (nos) gene, from A. tumefaciens,
- nptII gene (neomycin phosphotransferase II) from E. coli


6. Brief description of the method used for the genetic modification:
A standard Agrobacterium tumefaciens based transformation system was used for transformation of spring oilseed rape cv. Westar.

7. If the recipient or parental plant is a forest tree species, describe ways and extent of dissemination and specific factors affecting dissemination:
Not applicable.

Experimental Release

1. Purpose of the release:
The specific purpose of the release is the evaluation of the performance of the added trait under field conditions for research purposes.

2. Geographical location of the site:
Municipalities of Eslöv, Svalöv, Klippan, Kristianstad, Grästorp and Vara.

3. Size of the site (m2):
Total Area will not exceed 15 ha (150 000 m2).

4. Relevant data regarding previous releases carried out with the same GM-plant, if any, specifically related to the potential environmental and human health impacts from the release:
No previous releases have been carried out.

Environmental Impact and Risk Management

Summary of the potential environmental impact from the release of the GMPts:
The genetically modified oilseed rape lines show an altered fatty acid composition in seeds towards very long-chain polyunsaturated fatty acids. The plant material also shows tolerance to the aminoglycoside type antibiotics kanamycin and neomycin in tissue culture.
The added traits are not likely to have a critical influence under field conditions on any important competitive factors such as pollen production/dispersal, fertility or compatibility, nectar production, seed dispersal or general fitness. Based on observations in the greenhouse the genetically modified oilseed rape lines do not differ from the parental control lines in their morphology, flowering time, germination rate or kernel weight. No difference regarding persistence in agricultural habitats or invasiveness in natural habitats or persistence in agricultural habitats compared to conventional oilseed rape is expected.
Considering the very low likelihood of the introduced traits to confer increased fitness or the GM oilseed rape lines in any other way to become more persistent than the parental Brassica napus plant material in agricultural habitats or more invasive in natural habitats, the overall impact is expected to be negligible.
Any effects from the nptII gene on non-target organisms are not expected and have not been reported from other field trials harbouring this trait. The nptII gene is already present in the environment, since it is derived from soil organisms.


Brief description of any measures taken for the management of risks:
An isolation distance of 500m to other Brassica crops will be observed. Planting and harvesting equipment will be cleaned on site to prevent the dispersal of GM seeds. There will be no oilseed rape or any other crossable crop cultivation on the release area the following four years after the release. Potential volunteers will be monitored and removed according to conventional agricultural practice. During the release the trial site will be monitored at defined intervals.

Summary of foreseen field trial studies focused to gain new data on environmental and human health impact from the release:
Not applicable.

Final report
-

European Commission administrative information

Consent given by the Competent Authority:
Not known
Notification

Other notifications from this source

17,670

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