Date of publication: March 9,
2006
Source:
http://gmoinfo.jrc.it/gmp_browse_geninf.asp
Notification number:
B/DE/05/166
Member State:Germany
Date of Acknowledgement:17/08/2005
Title of the Project:
Influences of growth of transgenic potatoes on soil quality
in fields.
Proposed period of release From:01/05/2006
To:31/10/2007
Name of the Institute(s) or Company(ies): Technische
Universität München, Center for Life and Food Sciences, Chair of
Plant Breeding;
3. Is the same GMPt release planned elsewhere in the
Community?
No
4 - Has the same GMPt been notified elsewhere by the same
notifier?
No
Genetically
modified plant
1. Complete name of the
recipient or parental plant(s)
|
Common Name
|
Family Name
|
Genus |
Species
|
Subspecies
|
Cultivar/breeding line
|
| potato
|
solanaceae |
solanum |
solanum tuberosum |
tuberosum |
baltica |
2. Description of the traits and characteristics which have
been introduced or modified, including marker genes and previous
modifications:
The amount of the endogenous zeaxanthinepoxydase (ZEP) was
reduced in potato tubers by sense (clone SR 48/00#17) and
antisense (clone SR 47/00#18) expression of a fragment of the
potato zep gene under the control of the globular starch
synthase (GBSS) promoter. ZEP oxidises zeaxanthin to
violoxanthin. After reduction of its activity in the transgenic
lines the amount of the carotenoid zeaxanthin increased. For
selection of the transgenic clones the nptII marker gene was
used.
Genetic
modification
3. Type of genetic
modification:
Insertion;
4. In case of insertion of genetic material, give the source
and intended function of each constituent fragment of the region
to be inserted:
The constituent zep fragment has a length of 1044 bp. The zep
fragment encodes a part of the zeaxanthin epoxidase which
catalyses the conversion of zeaxanthine to violaxanthin.The zep
fragment was isolated by PCR from S. tuberosum cv. Desiree and
ligated into the TA cloning vector pCR2.1. From there it was
transferred by BamHI/Xbal-restriction into the vector pPGB121S.
pPGB121S was constructed by cloning a 0,8kb promoter fragment of
GBSS into pBI101 which derives from the binary vector pBIN19.
Two constructs were built that way. In one, zep was linked in
sense, in the other zep was linked in antisense-orientation to
the GBSS promoter resulting in pPGBzep and pPGBzep-antisense,
respectively.
6. Brief description of the method used for the genetic
modification:
For transformation Agrobacterium tumefaciens strain LBA 4404
was used. The selection of transformed tissue was performed on
kanamycin containing medium.
7. If the recipient or parental plant is a forest tree
species, describe ways and extent of dissemination and specific
factors affecting dissemination:
Not applicable.
Experimental
Release
1. Purpose of the release:
This project focusses on biological safety research, namely
on the influence of the transgenic potatoes on agricultural soil
organisms. In addition, plants and tubers produced will be
tested on whether they are identical to wildtype plants in
characters other than the zeaxanthin content. Furthermore, it
will be examined to which extent pollen of the modified plants
will fertilize other potato cultivars.
2. Geographical location of the site:
The experiment will be performed on two locations in Upper
Bavaria:
1. TUM experimental station Roggenstein, 82223 Olching, district
Fürstenfeldbruck, Flurstück 740/5.
2. A farm site at Oberviehhausen, 94574 Wallerfing, district
Deggendorf, Flurstück 1458
3. Size of the site (m2):
Area with transgenic plants: 324 m²
4. Relevant data regarding previous releases carried out with
the same GM-plant, if any, specifically related to the potential
environmental and human health impacts from the release:
In a previous release (B/DE/02/135) during a three years
period (2003-2005) using the same transgenic lines no effects on
the environment were observed. No effects on human health were
monitored either.
Environmental
Impact and Risk Management
Summary of the potential
environmental impact from the release of the GMPts:
Potato has no sexually compatible relatives in Germany. Thus,
an unexpected distribution in the environment is not possible.
Furthermore, potatoes are vegetatively propagated. Seedlings
from volunteer seeds will be destroyed by frost. There is no
obvious advantage in terms of persistence, invasiveness or
distribution been mediated by the transgene.
Brief description of any measures taken for the management of
risks:
1. The whole area is fenced and marked by respective signs.
2. Cleaning of the planting and harvesting tools on the
experimental plot.
3. Planting of 15m Sinapis arvensis around the experiment.
4. No other potatoes in a distance of 850 m.
5. Picking of those flowers from the transgenic plants where no
berries are to be harvested for quality tests and seed
production (for measuring outcrossing rates).
6. Non-used plants and tubers are macerated on the experimental
plot or autoclaved.
Summary of foreseen field trial studies focused to gain new
data on environmental and human health impact from the release:
The experiments shall give answers to the following
questions:
1. Influence on microbial populations in the rhizosphere and
residuesphere.
2. Influence of the transgenic characters on other metabolites
in different plant organs.
3. Influence on a rotation with winter wheat.
4. Influence on the degradation by microbes after growth of the
transgenic potatoes.
5. Comparison of N metabolism of transgenics and controls.
6. Estimation of outcrossing rates to neighbouring rows in the
experimental plots.
Final report
-
European
Commission administrative information
Consent given by the Competent
Authority: Not Known |
