Date of publication: March 9,
2006
Source:
http://gmoinfo.jrc.it/gmp_browse_geninf.asp
Notification number: B/DE/05/176
Member State:Germany
Date of Acknowledgement:21/11/2005
Title of the Project:
Assessment of technological properties, potential influence
on the environment, ingredients and specific characteristic of
different transgenic potatoes with pharmaceutical and technical
traits.
Proposed period of release From:01/05/2006
To:31/10/2008
Name of the Institute(s) or Company(ies): University
of Rostock;
3. Is the same GMPt release planned elsewhere in the
Community?
No
4 - Has the same GMPt been notified elsewhere by the same
notifier?
No
Genetically
modified plant
1. Complete name of the
recipient or parental plant(s)
|
Common Name
|
Family Name
|
Genus |
Species
|
Subspecies
|
Cultivar/breeding line
|
| potato
|
solanaceae |
solanum |
solanum tuberosum |
tuberosum |
desiree, albatros |
2. Description of the traits and characteristics which have
been introduced or modified, including marker genes and previous
modifications:
Genes for either vp60 (viral protein 60 of rabbit
haemorraghic disease virus, RHDV), ctxB (non-toxic B subunit
cholera toxin of Vibrio cholerae), PsbY-cyel (cyanophycin
synthetase) of Thermosynechococus elongates (with chloroplast
transit peptide from A. thaliana) and/ or nptII (neomycin
phosphotransferase II gene of E.coli) were introduced into
potato under control of the constitutive 35S promoter from
Cauliflower mosaic virus (CaMV).
Genetic
modification
3. Type of genetic
modification:
Insertion;
4. In case of insertion of genetic material, give the source
and intended function of each constituent fragment of the region
to be inserted:
VP60: constitutive 35S promoter (of Cauliflower mosaic virus
CaMV) driving expression of vp60 gene, vp60 gene (of RHDV)
encoding the viral protein VP60 which is the predominant protein
of the RHDV, t35S terminator (of CaMV)controlling transcription
termination of the vp60 gene, constitutive 35S promoter (of
CaMV) driving expression of nptII gene, nptII gene from E.coli
which confers kanamycin resistance to transformed cells, t35S
terminator (of CaMV)controlling transcription termination of the
nptII gene.
CTB: constitutive 35S promoter (of CaMV) driving expression of
ctxB gene, ctxB gene (of V. cholera) encoding the non-toxic B
subunit of the cholera toxin, which can interact with the
GM1-ganglioside receptor of mammalian cells and mediates
transport of the protein complex into the cell, t35S terminator
(of CaMV)controlling transcription termination of the ctxB gene,
constitutive 35S promoter (of CaMV) driving expression of nptII
gene, nptII gene which confers kanamycin resistance to
transformed cells, t35S terminator (of CaMV) controlling
transcription termination of the nptII gene.
Cyanophycin: constitutive 35S promoter (of CaMV) driving
expression of PsbY-cyel gene, PsbY-cyel gene (of
Thermosynecococcus elongatus with chloroplast transit peptide
isolated from Arabidopsis thaliana) encoding the cyanophycin
synthetase which is transported to the chloroplast to produce a
copolymer of L-aspartic acid and L-arginine, t35S terminator (of
CaMV) controlling transcription termination of the PsbY-cyel
gene, constitutive 35S promoter (of CaMV) driving expression of
nptII gene, nptII gene which confers kanamycin resistance to
transformed cells, t35S terminator (of CaMV)controlling
transcription termination of the nptII gene.
Neomycin Phosphotransferase II: constitutive 35S promoter (of
CaMV) driving expression of nptII gene, nptII gene from
Escherichia coli which confers kanamycin resistance to
transformed cells, t35S terminator (of CaMV) controlling
transcription termination of the nptII gene
6. Brief description of the method used for the genetic
modification:
An Agrobacterium-mediated transformation and plant
regeneration system slightly modified according to HORSCH et al.
1985 (Horsch, R., Fry, J., Hoffmann, N.L., Eichholtz, D.,
Rogers, S.G., Fraley, R.T. (1985) A simple and general method
for transferring genes into plants. Science 227:1229-1231) was
used for the transformation of potatoes cultivars Desiree and
Albatros. Transformed tissue was selected using kanamycin.
7. If the recipient or parental plant is a forest tree
species, describe ways and extent of dissemination and specific
factors affecting dissemination:
not applicable
Experimental
Release
1. Purpose of the release:
Evaluation of the new technological properties and
ingredients of the genetically modified potatoes with focus of
- expression of the transgenes in field grown potatoes
- environmental impact of transgene expression
- nutritional requirement
- volunteerism
- interaction with aphids
- nutritional physiology .
2. Geographical location of the site:
Groß Lüsewitz, Schlag 3/07, 18190 Sanitz, District Bad
Doberan (Mecklenburg/Vorpommern), Germany,
3. Size of the site (m2):
2006: 832 m2
2007: 2176 m2
2008: 1584 m2
4. Relevant data regarding previous releases carried out with
the same GM-plant, if any, specifically related to the potential
environmental and human health impacts from the release:
no previous release
Environmental
Impact and Risk Management
Summary of the potential
environmental impact from the release of the GMPts:
There is no scientific reason to assume that the transgenic
modifications resulting in a biosynthesis of the viral protein
VP60, of the non-toxic B subunit of the cholera toxin or of
cyanophycin may lead to changes in reproduction, dispersion,
persistence or invasiveness of these plants compared to
conventional potato plants. On the basis of current experience,
the transgenic plants do not differ from non-transgenic plants
in growth, size, phenology and seed formation. Under out-door
conditions there is no selective pressure that might lead to a
selective advantage of any of the transgenic lines. Due to
current experience no effect of the proteins in the plants on
pest or beneficial organisms or on health and environment is
expected.
Brief description of any measures taken for the management of
risks:
The measures for risk control are put down in a monitoring
plan. In order to avoid cross-pollination, the distance of the
nearest cultivation of potato plants will be at least 150 m.
Seed containing berries will be collected, removed and
autoclaved.
Sowing and harvesting machinery will be cleaned on site to
prevent the dispersal of GM material. Harvested plant material
will be transported from the site in closed and labelled
containers to the laboratories for analyses. Before harvest,
above ground parts will be inactivated chemically. Vegetative
and non reproductive plant material will be chopped and dragged
into the soil. The area will be controlled for volunteers during
the vegetation period following the release. In case volunteers
are identified, the monitoring will be extended for another
year. During the release period the field manager and trained
personnel will monitor the trial site at defined intervals.
Summary of foreseen field trial studies focused to gain new
data on environmental and human health impact from the release:
The field trials are designed to investigate the
environmental impacts:
- whether the cyanophycin production changes the nitrogen needs
of the plants
- whether the new features change the volunteer characteristics
of the plants
- whether there are any effects on the susceptibility for plant
pathogens (viruses, aphids).
Final report
-
European
Commission administrative information
Consent given by the Competent
Authority: Not Known |
