Alexandria, Virginia
February 28, 2006
Source:
US Patent Office
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United States Patent
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7,005,263
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Steimer , et al. |
February 28, 2006
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ABSTRACT
The invention relates to gene
silencing as observed after integration of transgenes into plant
genomes. Comparison of transcriptional gene expression between
an Arabidopsis line carrying a silent transgene present
in multiple copies and its mutant derivative mom1 impaired in
silencing of the transgene revealed two cDNA clones which are
expressed in the mutant plants, but not in the parental and not
in wild type plants. Both clones are derived from the same
family of transcripts referred to as TSI (Transcriptionally
Silent Information). Genomic templates encoding TSI are
repetitive elements with mainly pericentromeric location and
conserved organization among various ecotypes. Transcriptional
silencing of the genomic TSI templates is specifically released
in the mutant. Transcription of TSI can be used as a marker to
identify a defective silencing pathway in a plant.
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Inventors: |
Steimer; Andrea (Zurich,
CH); Scheid; Ortrun Mittelsten (Basel, CH); Paszkowski;
Jerzy (Nenzlingen, CH) |
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Assignee: |
Syngenta Participations AG
(Basel, CH) |
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Appl. No.: |
088384 |
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Filed: |
September 14, 2000 |
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PCT Filed: |
September 14, 2000
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PCT NO: |
PCT/EP00/08994
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371 Date: |
March 15, 2002
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102(e) Date: |
March 15, 2002
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PCT PUB.NO.: |
WO01/20010 |
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PCT PUB. Date:
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March 22, 2001 |
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