Date of publication: May 17,
2006
Source:
http://gmoinfo.jrc.it/gmp_browse_geninf.asp
Notification number:
B/DE/05/170
Member State:Germany
Date of Acknowledgement:03/11/2005
Title of the Project:
Deliberated release of genetically modified marker-free
amylopectin-potatoes in practice-oriented scale
Proposed period of release From:01/04/2006
To:31/10/2015
Name of the Institute(s) or Company(ies): Bavarian
State Research Center for Agriculture, Institute for Crop
Science and Plant Breeding;
3. Is the same GMPt release planned elsewhere in the
Community?
Yes: Germany
4 - Has the same GMPt been notified elsewhere by the same
notifier?
Yes
If yes, notification number(s):
B/DE/03/155
Genetically
modified plant
1. Complete name of the
recipient or parental plant(s)
|
Common Name
|
Family Name
|
Genus |
Species
|
Subspecies
|
Cultivar/breeding line
|
| potato
|
solanaceae |
solanum |
solanum tuberosum |
tuberosum |
Walli
|
2. Description of the traits and characteristics which have
been introduced or modified, including marker genes and previous
modifications:
Marker-free trait sequences comprising 581 base pairs (bp) of
exon 2 of the granular bound starch synthase gene (GBSS) from
potato inserted in anti-sense orientation under transcriptional
control of 5'-regulatory GBSS sequences were transferred into
potato using agrobacteria. Transcription of anti-sense GBSS is
terminated by terminator sequences from Cauliflower Mosaic Virus
(CaMV). Notably, selection genes have not been transferred.
Anti-sense GBSS sequences lead to a modified starch composition
in potato tubers and enrichment of amylopectin.
Genetic
modification
3. Type of genetic
modification:
Insertion;
4. In case of insertion of genetic material, give the source
and intended function of each constituent fragment of the region
to be inserted:
The transferred genetic material (T-DNA of pMFlp9-1) is
surrounded by right and left border sequences (RB, nopaline
type, 77 bp; LB, octopine type, 216 bp) from pPCV002 to promote
agrobacterial gene transfer. It contains 805 bp 5'-regulatory
GBSS sequences for a tuber enriched expression, exon 1 (11bp)
and intron 1 (220 bp) GBSS sequences as transcriptional
enhancers followed by 581 bp GBSS exon 2 sequences in anti-sense
orientation to inhibit GBSS and to modify the starch content of
the tuber tissue thereby. 162 bp terminator sequences from CaMV
35S locus are included to terminate anti-sense GBSS
transcription.
6. Brief description of the method used for the genetic
modification:
A novel marker-free binary T-DNA vector pMFlp9-1 was
constructed using standard molecular cloning methods. The binary
plasmid pMFlp9-1 was transferred into agrobacteria
(GV3101/pMP90RK) via electroporation. Internodial tissue from 4
weeks in vitro grown tip cultures of potato cultivar Walli was
incubated with cell suspensions of GV3101/pMP90RK/pMFlp9-1.
Regeneration of treated plant tissue was done using standard
plant tissue culture techniques. Selection procedures were not
performed. Over a period of 3 to 16 weeks after inoculation
regenerated shoots were harvested. Agrobacteria were removed
using Cefotaxim during regeneration and in vitro growth.
Approximately 5000 plants were generated. In vitro induced mini
tubers were treated with iodine solution. Line #1332 showed a
modified starch content.
Experimental
Release
1. Purpose of the release:
Deliberated release is performed (1) to verify results from
in vitro cultures and green house grown plants of line #1332
concerning the modified starch content of marker-free asGBSS
potatoes, (2) to analyse genetic stability and hypothetical
epigenetic effects under field conditions (case specific
monitoring) and (3) to characterise the potential industrial
applicability using practice-oriented parameter. Moreover,
general monitoring (e.g. plant development), monitoring of
volunteers from tubers and seeds, glycoalkaloid measurements and
frequency of out crossing will be accomplished. Effects on the
microbial diversity of the soil will be monitored.
2. Geographical location of the site:
Flur “Platschnwiesen” and “Jägerbruck”, 85290 Geisenfeld and
85084 Reichertshofen, Bavaria, Germany, Europe, and further
locations according to the simplified procedure (EU-Decision
94/730/EEC)
3. Size of the site (m2):
320000 m2
4. Relevant data regarding previous releases carried out with
the same GM-plant, if any, specifically related to the potential
environmental and human health impacts from the release:
Markerfree #1332 amylopectin-potatoes have been released
before (B/DE/03/155). General breeding properties and control
parameters of molecular analyses did not differ from the
unmodified cultivar Walli but in only the desired trait. After
two years under field conditions plants do stably express the
amylopectin enriched starch phenotype. A potential risk for the
environment or human health could not be detected.
Environmental
Impact and Risk Management
Summary of the potential
environmental impact from the release of the GMPts:
Note especially if the introduced traits could directly or
indirectly confer an increased selective advantage in natural
environments; also explain any significant expected
environmental benefits
The genetically modified potato plant #1332 carries GBSS
sequences from potato on agrobacterial T-DNA and does not
harbour any antibiotic or herbicide selection gene. The
introduced DNA does not encode new proteins, but inhibits
endogenous GBSS leading to modified starch quality of the
tubers. Therefore, selection advantages due to the genetic
modification are not evident. Increased persistence of
marker-free plants in agronomic regions or an enhanced potency
to invade natural areas is not expected, as transferred
sequences can be already found in potato. To our present
knowledge, spreading antibiotic resistance genes and any other
possible harmful effects on the environment can be excluded in
deliberated release experiments with the marker-free potato line
#1332. Moreover, significant environmental benefits would be
expected during industrial amylopectin production from such
tubers. Large amounts of alkaline solutions and a huge quantity
of waste water could be avoided.
Brief description of any measures taken for the management of
risks:
The biological safety of potato is high for following
reasons. Potato is not a native plant in Europe and does not
pollinate any wild species in this area. Potato is self and
insect pollinating having pollen with a very low dispersal
range. No potato volunteers can be found outside agronomic
regions. Nevertheless, a case specific monitoring towards
stability of the transgene, a general monitoring according to
good breeding practice, monitoring of volunteers of tubers or
seeds and examinations to describe the glycoalkaloid content
will be performed. Appropriate plant production techniques are
applied (e.g. two rows of pollen acceptor plants, special soil
treatment to minimise seed volunteers). In post-harvest
inquiries any remaining transgenic potato volunteers on the
release site will be removed, chopped and composted.
Summary of foreseen field trial studies focused to gain new
data on environmental and human health impact from the release:
A monitoring plan according to directive 2001/18/EC is
performed. General and case specific analyses are foreseen.
Among the most important features will be a detailed description
of line #1332 according to good breeding practice, examination
of transgene stability of marker-free potatoes and their
breeding products, quantification of the number of volunteers
from tubers or seeds and inspection of glycoalkaloid content in
tubers.
Final report
-
European
Commission administrative information
Consent given by the Competent
Authority: Not Known |
