Date of publication: January 16,
2006
Source:
http://gmoinfo.jrc.it/gmp_browse_geninf.asp
Notification number:
B/CZ/05/03
Member State: Czech Republic
Date of Acknowledgement: 29/12/2005
Title of the Project:
Release into the environment of genetically modified potatoes
with the change of sugar content in tubers
Proposed period of release From:01/04/2006
To:30/09/2010
Name of the Institute(s) or Company(ies): Vesa
Velhartice and Institute of Experimental Botany, Czech Academy
of Sciences;
3. Is the same GMPt release planned elsewhere in the
Community?
No
4 - Has the same GMPt been notified elsewhere by the same
notifier?
No
Genetically
modified plant
1. Complete name of the
recipient or parental plant(s)
|
Common Name
|
Family Name
|
Genus |
Species
|
Subspecies
|
Cultivar/breeding line
|
| potato
|
solanaceae |
solanum |
solanum tuberosum |
tuberosum |
|
2. Description of the traits and characteristics which have
been introduced or modified, including marker genes and previous
modifications:
Trait: increased metabolism of reducing sugars in tubers
Marker gene: nptII gene (tolerance to kanamycin) as selectable
marker gene to identify transgenic cells in tissue culture
Genetic
modification
3. Type of genetic
modification:
Insertion;
4. In case of insertion of genetic material, give the source
and intended function of each constituent fragment of the region
to be inserted:
- T-DNA borders, pTiT37, for incorporation into plant
chromosome
- nptII gene, E. coli, kanamycin tolerance in plant material
- Promoter and terminator from nopaline synthase gene, A.
tumefaciens, gene regulation
- Promoter of patatin gene pB33, S. tuberosum, gene regulation,
and terminator from octopine synthase gene, A. tumefaciens, gene
regulation
- Lbpfk gene, Lactobacillus bulgaricus, bacterial
phosphofructokinase
6. Brief description of the method used for the genetic
modification:
Plasmid DNA was introduced into the potato lines by
Agrobacterium-mediated gene transfer technology. This is
standard technology for potato transformation.
7. If the recipient or parental plant is a forest tree
species, describe ways and extent of dissemination and specific
factors affecting dissemination:
Not applicable
Experimental
Release
1. Purpose of the release:
The purpose of the release is, to compile data on agronomical
performance, as well as to collect plant material for sugar
content analysis, as well as to generate seed tubers.
2. Geographical location of the site:
Velhartice (Plzensky region)
3. Size of the site (m2):
less than 1 ha
4. Relevant data regarding previous releases carried out with
the same GM-plant, if any, specifically related to the potential
environmental and human health impacts from the release:
There has been previous release of potato lines transformed
with the same constructs in the Czech Republic. No adverse
impacts on the environment or human health have been recorded in
any of the trials.
Environmental
Impact and Risk Management
Summary of the potential
environmental impact from the release of the GMPts:
The genetically modified potato lines show decrease in
reducing-sugar content in cold-stored tubers. No difference
regarding persistence in agricultural habitats or invasiveness
in natural habitats compared to conventional potato varieties is
expected. Nor is it expected that a selective advantage or
disadvantage is conferred to the GM lines via the introduced
traits. Due to the measures to be taken during the release
(distance to or absence of cultivated and wild relatives) the
potential for gene transfer will be virtually excluded.
Interactions of the GM potato lines with non-target species and
resulting effects will be comparable to those of conventional
potato varieties. No toxic or allergenic effects are expected
from bacterial phosphofructokinase (located in tubers mainly),
nor from the neomycin phosphotransferase. Measures in place
under current field trial practice will protect the trial from
damage by animals as well as safeguard that all seed and plant
material is properly managed, harvested, stored, transported or
disposed of to minimize or prevent contact with humans or
animals. No effects are expected on biogeochemical processes.
The GM potato lines will be cultivated under conventional
agricultural practices.
Brief description of any measures taken for the management of
risks:
An isolation distance of 10 m to other potato varieties will
be observed. Planting and harvesting equipment will be cleaned
on site to prevent the dispersal of GM tubers. There will be no
potato cultivation on the release area the year following the
release. Potential volunteers will be monitored and removed
according to conventional agricultural practice. During the
release the trial site will be monitored at defined intervals.
Final report
-
European
Commission administrative information
Consent given by the Competent
Authority: Not Known |
