Date of publication: February 14,
2006
Source:
http://gmoinfo.jrc.it/gmp_browse_geninf.asp
Notification number:
B/ES/06/29
Member State:Spain
Date of Acknowledgement:04/01/2006
Title of the Project:
Official trials for registration of maize varieties resistant
to certain Lepidopteran insect pests
Proposed period of release From:01/04/2006
To:30/11/2006
Name of the Institute(s) or Company(ies): Dow
AgroSciences;
3. Is the same GMPt release planned elsewhere in the
Community?
No
4 - Has the same GMPt been notified elsewhere by the same
notifier?
No
Genetically
modified plant
1. Complete name of the
recipient or parental plant(s)
|
Common Name
|
Family Name
|
Genus |
Species
|
Subspecies
|
Cultivar/breeding line
|
| maize
|
poaceae |
zea
|
zea
mays |
mays
|
|
2. Description of the traits and characteristics which have
been introduced or modified, including marker genes and previous
modifications:
The cry1F(synpro) and pat genes were inserted into the maize
genome to express the Cry1F and PAT proteins, aiming at
conferring resistance to certain lepidopteran insect pests and
tolerance to glufosinate ammonium herbicide into the maize
plants.
Genetic
modification
3. Type of genetic
modification:
Insertion;
4. In case of insertion of genetic material, give the source
and intended function of each constituent fragment of the region
to be inserted:
The genetic material in PHI8999A intended to be inserted into
the maize genome contains a synthetic version of the cry1F and
pat genes, along with the regulatory signals required for the
transcription and the expression of these genes into the plant
tissues.
The cry1F gene (1818 bp. Origin Bacillus thuringiensis subs.
aizawai) is driven by the UbiZm1 promoter (1986 bp, origin zea
maiz) followed by the transcription terminator sequence ORF25
PolyA (714 bp, origin Agrobacterium tumefasciens pTi15995). The
function of the Cry1F protein in 1507 maize is to confer
resistance against some lepidopteran insect pests including
Sesamia spp.
The pat gene (552 bp; origin: Streptomyces viridochromogenes
strain Tü494) is under the control of the CaMV35S promoter and
terminator (554 y 204 bp, respectively; origin: mosaic
cauliflower). The function of the PAT protein is to confer
tolerance to maize 1507 against glufosinate ammonium herbicide.
6. Brief description of the method used for the genetic
modification:
The genes were introduced into the maize genome by using the
particle bombardment technique with the linear fragment
containing the genes cry1F and pat genes along with the
regulatory sequences for their expression (PHI8999A).
7. If the recipient or parental plant is a forest tree
species, describe ways and extent of dissemination and specific
factors affecting dissemination:
Not relevant.
Experimental
Release
1. Purpose of the release:
The purpose of the release is to determine the field efficacy
of the Cry1F and PAT proteins expressed in transgenic maize 1507
against some lepidopteran insect pests under natural infestation
conditions. The Cry proteins are known to be very specific
against certain lepidopteran insects and no effects on
non-target organisms are expected.
No changes on survivability, weediness or dissemination were
observed in the field trials performed during the years 2000 and
2001 in the US, Argentina, South Africa and Brazil when compared
with conventional maize varieties as a consequence of the
expression of Cry1F or PAT proteins in maize 1507.
2. Geographical location of the site:
Aragón (8 locations):
- Zaragoza: 2 locations in Montańana, Cogullada, Monreal de
Ariza , Ejea de los Caballeros.
- Huesca: 2 locations in Gurrea de Gállego, Poleńino.
Navarra (1 location): Monteagudo.
Sevilla (1 location): Alcalá de Guadaira.
La Rioja (1 location): Alfaro.
3. Size of the site (m2):
Aragón: 22940 m2.
Navarra: 3500 m2.
Sevilla: 4000 m2.
La Rioja: 1150 m2.
4. Relevant data regarding previous releases carried out with
the same GM-plant, if any, specifically related to the potential
environmental and human health impacts from the release:
During the field trials performed in the US, Argentina,
Brazil, South Africa, no particular characteristics that could
have a negative impact on the environment or human health were
observed. Moreover, no significant differences between the maize
1507 and the conventional maize plants were detected.
Environmental
Impact and Risk Management
Summary of the potential
environmental impact from the release of the GMPts:
No maize sexual compatible species have been found in Spain.
Additionally, the field trials will be isolated from any other
maize field by at least 60 m. Maize has a long history of safe
use and been extensively domesticated by humans. The genes
introduced into the maize 1507, cry1F and pat have a very
specific mode of action, the first one conferring resistance to
some lepidopteran insect pests and the latter conferring
tolerance to the ammonium glufosinate herbicide. With the
adoption of this technology the insecticide will be localised
within the transgenic plants, reducing the environmental impact
to surrounding areas and the farmers will reduce fuel costs and
soil compaction through a substantial reduction in the numbers
of applications required to control insect pests.
Brief description of any measures taken for the management of
risks:
The trials are going to be isolated from any other maize plot
by a minimum distance of 60m. A non-GMO maize 4 row border will
be planted and subsequently destroyed at the end of the
experiment. The following year a different crop will be planted
in these lots to facilitate the detection and destruction of any
volunteer plant. All the plant products from these trials will
be manually or chemically destroyed. Trained people will carry
on the experiment and follow up closely. Regular monitoring
visits will be implemented during the growing season to evaluate
the performance and looking for any unintended effects and in
the following season to control volunteers.
Summary of foreseen field trial studies focused to gain new
data on environmental and human health impact from the release:
These field trials are implemented to assess the efficacy of
the Cry1F protein expressed in maize 1507 against certain
lepidopteran insect pests and the PAT protein conferring
tolerance against glufosinate ammonium herbicide. According to
the results obtained in the trials, the commercial strategy will
be drawn.
Final report
-
European
Commission administrative information
Consent given by the Competent
Authority: Not Known |
