Date of publication: October 17,
2005
Source:
http://gmoinfo.jrc.it/gmp_browse_geninf.asp
Notification number:
B/NL/05/04
Member State:Netherlands
Date of Acknowledgement:08/09/2005
Title of the Project:
Application for the release into the environment of potatoes
with altered starch composition (I) according to the Genetically
Modified Organisms Decree
Proposed period of release From:01/01/2006
To:31/12/2010
Name of the Institute(s) or Company(ies): BASF Plant
Science GmbH;
3. Is the same GMPt release planned elsewhere in the
Community?
Yes: Germany; Sweden
4 - Has the same GMPt been notified elsewhere by the same
notifier?
Yes
If yes, notification number(s):
B/SE/03/1946; B/SE/04/1101
Other notifications
Consent B/SE/04/1101 covers potato lines transformed with
construct pHAS3. Consent B/SE/03/1946 covers potato lines
transformed with construct pHASHS5.
Genetically
modified plant
1. Complete name of the
recipient or parental plant(s)
|
Common Name
|
Family Name
|
Genus |
Species
|
Subspecies
|
Cultivar/breeding line
|
| potato
|
solanaceae |
solanum |
solanum tuberosum |
tuberosum |
P763
and P800 |
2. Description of the traits and characteristics which have
been introduced or modified, including marker genes and previous
modifications:
-increased accumulation of amylose starch in tubers
-marker gene ahas (tolerance to Imidazolinones) as selection
gene to identify transgenic cells in tissue culture
Genetic
modification
3. Type of genetic
modification:
Insertion;
4. In case of insertion of genetic material, give the source
and intended function of each constituent fragment of the region
to be inserted:
-T-DNA borders, pTiT37, for incorporation into plant
chromosome.
-Recombinant ahas gene, A. thaliana, imidazolinone tolerance in
plant material.
-Promoter and terminator from nopaline synthase gene, A.
tumefaciens, gene regulation.
-Promoter of gbss gene, S. tuberosum, gene regulation
-the inverted repeat fragments of the be1 and be2 genes leads to
a reduction of the amylopectin fraction in the starch of the
potato tuber resulting in a concomitant increase in the levels
of amylose
- Starch biosynthesis enhancing protein 1, S. tuberosum,
increase in starch content in amylose potatoes
6. Brief description of the method used for the genetic
modification:
Plasmid-derived DNA was introduced into the potato lines by
Agrobacterium-mediated gene transfer technology
7. If the recipient or parental plant is a forest tree
species, describe ways and extent of dissemination and specific
factors affecting dissemination:
-
Experimental
Release
1. Purpose of the release:
- Evaluate the quality of starch composition under field
conditions
- Investigate the general agricultural value of the modified
lines
- Produce seed potatoes for future trials
2. Geographical location of the site:
The location of one release site will be at Borger-Odoorn and
the location of the second release site will in Lingewaard
3. Size of the site (m2):
10.000 m2 per site maximally will be planted annually
4. Relevant data regarding previous releases carried out with
the same GM-plant, if any, specifically related to the potential
environmental and human health impacts from the release:
There have been previous releases of potato lines transformed
with the same constructs in several countries. No adverse
impacts on the environment or human health have been recorded in
any of the trials.
Environmental
Impact and Risk Management
Summary of the potential
environmental impact from the release of the GMPts:
The genetically modified potato lines contain be1 and be2
gene fragments as inverted repeat. Their expression leads to a
reduced amylopectin accumulation in tubers, resulting in a
concomitant increase in amylose content. All greenhouse and
field observations so far confirm that the behaviour of the
modified lines is identical to that of the recipient plant
material. Field trials with constructs containing the same be
gene fragments have been conducted since 2000.
The ahas gene expressed in the potato plants imparts tolerance
to the herbicidal active substance Imazamox to the shoots during
the selection process in cell culture. This confers no selective
advantage in the field since Imidazolinone herbicides are not
approved for use on crops in NL and no field tolerance is
expected in the potato plants. No difference with respect to
persistence in agriculturally utilised habitats or invasiveness
into natural habitats as compared to conventional potato
varieties is expected. Through the measures that are taken
during the release, distance from or absence of conventionally
cultivated potatoes or wild species, the possibility of any gene
transfer can be virtually ruled out. Even in the very improbable
event that pollen were to be transferred to genetically
unmodified potato plants, no consequences are to be expected,
since potato propagation conventionally takes place via tubers
and not via seeds. The interactions of the genetically modified
potato line with non-target organisms and the effects resulting
from this will be comparable to those with conventional potato
varieties.
Brief description of any measures taken for the management of
risks:
-isolation distance: 10 m
-careful harvest to avoid volunteers
-transportation in specific vehicles and closed containers
-crop rotation: no potato crop the following year
-destruction of waste material
Final report
European
Commission administrative information
Consent given by the Competent
Authority: Not Known |
