Date of publication: March 31,
2005
Source:
http://gmoinfo.jrc.it/gmp_browse_geninf.asp
Notification number: B/CZ/05/642
Member State: Czech Republic
Date of Acknowledgement:02/03/2005
Title of the Project: Release into the environment of
genetically modified potatoes with an altered starch composition
Proposed period of release From:01/04/2005
To:31/10/2009
Name of the Institute(s) or Company(ies): BASF (Czech)
spol. s r.o.;
3. Is the same GMPt release planned elsewhere in the
Community?
No
4 - Has the same GMPt been notified elsewhere by the same
notifier?
Yes
If yes, notification number(s):
B/DE/03/153; B/DE/03/154; B/NL/03/09; B/NL/03/10; B/SE/95/28;
B/SE/96/530; B/SE/97/1782; B/SE/99/1087
Other notifications
22-1104/02 and 22-1019/02 (by Plant Science Sweden AB in
Sweden), B/DE/03/154, B/DE/03/153, B/NL/03/10, B/NL/03/09 (BASF
Plant Science GmbH), 22-4314/92, 22-4363/93, 22-28/95,
22-530/96, 22-1782/97, 22-2519/98, 22-1087/99, 22-1019/00 (by
Amylogene HB and Plant Science Sweden AB).
Genetically
modified plant
1. Complete name of the
recipient or parental plant(s)
|
Common Name
|
Family Name
|
Genus |
Species
|
Subspecies
|
Cultivar/breeding line
|
| potato
|
solanaceae |
solanum |
solanum tuberosum |
tuberosum |
|
2. Description of the traits and characteristics which have
been introduced or modified, including marker genes and previous
modifications:
A gene modifying the starch composition in potato tubers
raising the level of amylopectin has been introduced into
potato. An acetohydroxyacid synthase gene (lines AM02-1003,
AM02-1005, AM02-1008, AM02-1010, AM02-1012, AM02-1014,
AM02-1017) or a neomycin phophotransferase II gene (reference
line EH92-527-1) have been used as selectable marker genes.
Genetic
modification
3. Type of genetic
modification:
Insertion;
4. In case of insertion of genetic material, give the source
and intended function of each constituent fragment of the region
to be inserted:
A gene containing the coding region of potato gbss in
antisense orientation relative to the promoter, flanked by the
the gbss promoter from Solanum tuberosum and the polyadenylation
sequence from Agrobacterium tumefaciens nopaline synthase gene
has been inserted into potato lines AM02-1003, AM02-1005,
AM02-1008, AM02-1010, AM02-1012, AM02-1014, AM02-1017 thus
reducing the amount of amylose in the starch fraction. An ahas
gene (acetohydroxyacid synthase) from Arabidopsis thaliana
flanked by the nos gene promoter and the octopine synthase
polyadenylation sequence from Agrobacterium tumefaciens serves
as selectable marker gene conferring tolerance to Imazamox.
In potato event EH92-527-1 a gene consisting of a potato gbss
(granule bound starch synthase) promoter, a fragment of the
coding region of potato gbss in antisense orientation relative
to the promoter and the polyadenylation sequence from
Agrobacterium tumefaciens nopaline synthase gene (gene construct
pHoxwG) have been inserted into potato thus reducing the amount
of amylose in the starch fraction. In addition the neomycin
phosphotransferase gene (nptII) connected to the Agrobacterium
tumefaciens nopaline synthase promoter and polyadenylation
signal has been inserted as selectable marker gene conferring
resistance to kanamycin.
6. Brief description of the method used for the genetic
modification:
Plasmid DNA was introduced into the potato lines by
Agrobacterium-mediated gene transfer technology. This is
standard technology for potato transformation.
7. If the recipient or parental plant is a forest tree
species, describe ways and extent of dissemination and specific
factors affecting dissemination:
Not applicable.
Experimental
Release
1. Purpose of the release:
The purpose of the release is, within the frame of safety
studies to compile data on agronomical performance and
environmental effects, as well as to collect plant material for
further analyses, as well as to generate seed tubers.
2. Geographical location of the site:
Humpolec (Vysocina region)
3. Size of the site (m2):
Approx. 10 000 m2
4. Relevant data regarding previous releases carried out with
the same GM-plant, if any, specifically related to the potential
environmental and human health impacts from the release:
No adverse impacts on the environment or human health have
been recorded for the potato lines in previous field trials in
Sweden, Germany and the Netherlands.
Environmental
Impact and Risk Management
Summary of the potential
environmental impact from the release of the GMPts:
The genetically modified potato lines show a shift of the
tuber starch composition to amylopectin. No difference regarding
persistence in agricultural habitats or invasiveness in natural
habitats compared to conventional potato varieties is expected.
Nor is it expected that a selective advantage or disadvantage is
conferred to the GM lines via the introduced traits. Due to the
measures to be taken during the release (distance to or absence
of cultivated and wild relatives) will the potential for gene
transfer virtually be excluded. Interactions of the GM potato
lines with non-target species and resulting effects will be
comparable to those of conventional potato varieties. No toxic
or allergenic effects are expected from elevated levels of
amylopectin, nor from the acetohydroxyacid synthase or neomycin
phosphotransferase II. Measures in place under current field
trial practice will protect the trial from damage by animals as
well as safeguard that all seed and plant material is properly
managed, harvested, stored, transported or disposed of to
minimize or prevent contact to humans or animals. No effects are
expected on biogeochemical processes. The GM potato lines will
be cultivated under conventional agricultural practices.
Brief description of any measures taken for the management of
risks:
An isolation distance of 5 rows to other potato varieties
will be observed. Planting and harvesting equipment will be
cleaned on site to prevent the dispersal of GM tubers. There
will be no potato cultivation on the release area the year
following the release. Potential volunteers will be monitored
and removed according to conventional agricultural practice.
During the release the trial site will be monitored at defined
intervals.
Summary of foreseen field trial studies focused to gain new
data on environmental and human health impact from the release:
Not applicable. |
