Date of publication: March 18,
2005
Source:
http://gmoinfo.jrc.it/gmp_browse_geninf.asp
Notification number: B/ES/05/17
Member State: Spain
Date of Acknowledgement:11/02/2005
Title of the Project:
Testing of maize resistant to certain Coleopteran and
Lepidopteran insects and tolerant to herbicides
Proposed period of release From:01/04/2005
To:31/12/2008
Name of the Institute(s) or Company(ies): PIONEER
HI-BRED SPAIN S.L.;
3. Is the same GMPt release planned elsewhere in the
Community?
No
4 - Has the same GMPt been notified elsewhere by the same
notifier?
No
Genetically
modified plant
1. Complete name of the
recipient or parental plant(s)
|
Common Name
|
Family Name
|
Genus |
Species
|
Subspecies
|
Cultivar/breeding line
|
| maize
|
poaceae |
zea
|
zea
mays |
mays
|
experimental varieties |
2. Description of the traits and characteristics which have
been introduced or modified, including marker genes and previous
modifications:
The genetically modified 59122x1507xNK603 maize varieties
that will be tested in these field trials are resistant to
certain Coleopteran insects, such as Western corn rootworm
(Diabrotica virgifera virgifera), and certain Lepidopteran
insects, such as European corn borer (Ostrinia nubilalis), and
can tolerate the use of glufosinate-ammonium and glyphosate
herbicides for the weeding of maize field. This maize was
derived through traditional breeding methods between progeny of
the genetically modified 59122 maize, 1507 maize and NK603 maize
(from Monsanto).
Genetic
modification
3. Type of genetic
modification:
Insertion;
4. In case of insertion of genetic material, give the source
and intended function of each constituent fragment of the region
to be inserted:
59122x1507xNK603 maize contains the following genetic
elements:
- the cry34Ab1 and cry35Ab1 genes from Bacillus thuringiensis
strain PS149B1 that confer resistance to certain Coleopteran
insects, such as Diabrotica virgifera virgifera, and the pat
gene from Streptomyces viridochromogenes that confers tolerance
to glufosinate-ammonium herbicide, from 59122 maize.
- the cry1F gene from Bacillus thuringiensis var. aizawai that
confers resistance to certain Lepidopteran insects, such as
Ostrinia nubilalis, and the pat gene from Streptomyces
viridochromogenes that confers tolerance to glufosinate-ammonium
herbicide, from 1507 maize.
- the cp4epsps genes from Agrobacterium sp. strain CP4 that
confer tolerance to glyphosate herbicide, from NK603 maize.
All these genes have been introduced in the corresponding maize
along with their regulatory components necessary for expression.
6. Brief description of the method used for the genetic
modification:
No genetic modification was made on the 59122x1507xNK603
maize varieties directly: they were derived through traditional
breeding methods between progeny of the genetically modified
59122, 1507 and NK603 maize.
59122 maize was genetically modified using Agrobacterium
transformation method. 1507 maize was genetically modified using
a particle acceleration method. NK603 maize was genetically
modified by Monsanto using a particle acceleration method.
7. If the recipient or parental plant is a forest tree
species, describe ways and extent of dissemination and specific
factors affecting dissemination:
Not applicable.
Experimental
Release
1. Purpose of the release:
Several types of trials are planned, to collect various maize
tissue samples for protein expression analysis, to evaluate the
impact of the cultivation of transgenic maize varieties on key
non-target arthropod populations, to control maize volunteers
with different herbicides, to evaluate the residual behavior of
glufosinate and glyphosate in transgenic maize, to collect data
on the agronomic value and insect resistance of transgenic maize
varieties.
2. Geographical location of the site:
The release is planned at the following locations in Spain in
2005:
- Aragón: Pastriz (Zaragoza), Gurrea de Gállego (Huesca).
- Cataluña: Alpicat (Lérida), Alguaire (Lérida).
- Madrid: Estremera (Madrid).
- Navarra: Fustiñana (Navarra)
- Castilla-La Mancha: Membrilla (Ciudad Real)
3. Size of the site (m2):
In 2005, each site in Aragón, Madrid, Navarra, Extremadura
and Castilla y Léon could have up to 1000 m² sown with the
genetically modified maize subject of this notification
(59122x1507xNK603 maize), the total trial surface (all varieties
and borders included) being larger at each site. In 2005, each
site in Cataluña could have up to 8500m² sown with the
genetically modified maize subject of this notification, the
total trial surface (all varieties and borders included) being
larger at each site.
In the following years, there could have up to 10000 m² of the
genetically modified maize subject of this notification sown per
site, the total trial surface at each site (all varieties and
borders included) being larger.
4. Relevant data regarding previous releases carried out with
the same GM-plant, if any, specifically related to the potential
environmental and human health impacts from the release:
Field trials have already been conducted at several locations
across maize growing regions in North America, South America and
Europe and no environmental problems were reported for these
trials. The transgenic plants were indistinguishable from the
non-genetically modified maize plants except for showing
resistance to certain Coleopteran insects, such as Western corn
rootworm, and certain Lepidopteran insects such as European corn
borer and showing tolerance when glufosinate and glyphosate
herbicides were applied, traits due to the genetic
modifications.
Environmental
Impact and Risk Management
Summary of the potential
environmental impact from the release of the GMPts:
No risks to human and animal health or the environment from
the deliberate release of genetically modified maize resistant
to certain Coleopteran and Lepidopteran insects and tolerant to
glufosinate and glyphosate herbicides are expected, as per
information contained in the environmental risk assessment
included in the notification.
Brief description of any measures taken for the management of
risks:
The shedding of pollen from the genetically modified plants
will be controlled by maintaining a 200-meter isolation distance
with any other non-experimental maize crop. In addition, the
trial site will be surrounded by four border rows of
conventional maize of a similar relative maturity that will be
also destroyed at the end of the release.
Seed dispersal of individual kernels does not generally occur.
They are fixed on a cob and enclosed in many husks that protect
the seeds from outside contact. When a few seeds need to be
collected for analysis; this will be done by sampling the whole
ear and unused seeds will be destroyed.
In case of any emergency, the trial could be stopped by
application of a non-selective herbicide, other than glufosinate
and glyphosate, or by mechanical destruction, and incorporation
into the soil.
At the end of the release, all remaining plant matter that has
not been harvested for analyses will be destroyed by chopping
and incorporating into the soil.
Volunteer maize will be monitored during the following year. A
herbicide treatment (other than glufosinate and glyphosate) will
be used in order to ensure destruction if needed.
There will be no commercial maize grown on the same field the
following year.
Summary of foreseen field trial studies focused to gain new
data on environmental and human health impact from the release:
Not applicable to this release. |
