Date of publication: March 18,
2005
Source:
http://gmoinfo.jrc.it/gmp_browse_geninf.asp
Notification number: B/ES/05/18
Member State: Spain
Date of Acknowledgement:11/02/2005
Title of the Project:
Testing of maize varieties resistant to certain Coleopteran
insects and tolerant to a herbicide
Proposed period of release From:01/04/2005
To:31/12/2008
Name of the Institute(s) or Company(ies): PIONEER
HI-BRED SPAIN S.L.;
3. Is the same GMPt release planned elsewhere in the
Community?
No
4 - Has the same GMPt been notified elsewhere by the same
notifier?
No
Genetically
modified plant
1. Complete name of the
recipient or parental plant(s)
|
Common Name
|
Family Name
|
Genus |
Species
|
Subspecies
|
Cultivar/breeding line
|
| maize
|
poaceae |
zea
|
zea
mays |
mays
|
experimental varieties |
2. Description of the traits and characteristics which have
been introduced or modified, including marker genes and previous
modifications:
New genes were introduced into the genetically modified 59122
maize plants so that they can fight against the damages caused
by certain Coleopteran insect pests, such as Western corn
rootworm (Diabrotica virgifera virgifera), and tolerate
application of glufosinate-ammonium herbicide for the weeding of
maize crop.
Genetic
modification
3. Type of genetic
modification:
Insertion;
4. In case of insertion of genetic material, give the source
and intended function of each constituent fragment of the region
to be inserted:
The genes introduced in 59122 maize are the cry34Ab1 and
cry35Ab1 genes from Bacillus thuringiensis strain PS149B1,
providing resistance to certain Coleopteran insects and the pat
gene from Streptomyces viridochromogenes providing tolerance to
glufosinate herbicide, together with only their regulatory
components necessary for expression in maize plant.
6. Brief description of the method used for the genetic
modification:
The genetic modification was done using Agrobacterium
transformation method to introduce the DNA insert into the maize
genome. The T-DNA on the Ti plasmid has been replaced by the
construction containing the 3 genes along with the regulatory
components necessary for expression.
7. If the recipient or parental plant is a forest tree
species, describe ways and extent of dissemination and specific
factors affecting dissemination:
Not applicable.
Experimental
Release
1. Purpose of the release:
Several types of trials are planned, to collect various maize
tissue samples for protein expression analysis, to evaluate the
impact of the cultivation of transgenic maize varieties on key
non-target arthropod populations, to collect data on the
agronomic value and insect resistance of transgenic maize
varieties.
2. Geographical location of the site:
The release is planned at the following locations in Spain in
2005:
- Aragón: Pastriz (Zaragoza), Gurrea de Gállego (Huesca).
- Cataluña: Alpicat (Lérida), Alguaire (Lérida).
- Madrid: Estremera (Madrid).
- Navarra : Fustiñana (Navarra)
- Castilla-La Mancha : Membrilla (Ciudad Real)
3. Size of the site (m2):
In 2005, each site in Aragón, Madrid, Navarra and Castilla-La
Mancha could have up to 100 m² sown with the genetically
modified maize subject of this notification (59122 maize), the
total trial surface (all varieties and borders included) being
larger at each site. In 2005, each site in Cataluña could have
up to 4100m² sown with the genetically modified maize subject of
this notification, the total trial surface (all varieties and
borders included) being larger at each site.
In the following years, there could have up to 6000 m² of the
genetically modified maize subject of this notification sown per
site, the total trial surface at each site (all varieties and
borders included) being larger.
4. Relevant data regarding previous releases carried out with
the same GM-plant, if any, specifically related to the potential
environmental and human health impacts from the release:
Many field trials have already been conducted at several
locations since 2001 and no environmental problems were reported
for these trials. The transgenic plants behaved as conventional
maize. They were indistinguishable from the non-genetically
modified maize plants except for showing resistance to certain
Coleopteran insects, such as the Western corn rootworm, and
showing tolerance when glufosinate has been applied, traits due
to the genetic modification.
Environmental
Impact and Risk Management
Summary of the potential
environmental impact from the release of the GMPts:
No risks to human and animal health or the environment from
the deliberate release of genetically modified maize resistant
to certain Coleopteran insects and tolerant to glufosinate
herbicide are expected, as per information contained in the
environmental risk assessment included in the notification.
Brief description of any measures taken for the management of
risks:
The shedding of pollen from the genetically modified plants
will be controlled by maintaining a 200-meter isolation distance
with any other non-experimental maize crop. In addition, the
trial site will be surrounded by four border rows of
conventional maize of a similar relative maturity that will be
also destroyed at the end of the release.
Seed dispersal of individual kernels does not generally occur.
They are fixed on a cob and enclosed in many husks that protect
the seeds from outside contact. When a few seeds need to be
collected for analysis; this will be done by sampling the whole
ear and unused seeds will be destroyed.
In case of any emergency, the trial could be stopped by
application of a non-selective herbicide other than glufosinate,
or by mechanical destruction, and incorporation into the soil.
At the end of the release, all remaining plant matter that has
not been harvested for analyses will be destroyed by chopping
and incorporating into the soil.
Volunteer maize will be monitored during the following year. An
herbicide treatment (other than glufosinate) will be used in
order to ensure destruction if needed.
There will be no commercial maize grown on the same field the
following year.
Summary of foreseen field trial studies focused to gain new
data on environmental and human health impact from the release:
Not applicable to this release. |
