Deliberate release into the E.U.
environment of GMOs for any other purposes than placing on the
market:
Official trials for registration of
maize varieties resistant to certain Lepidopteran insect pests -
Dow AgroSciences - Spain |
Date of publication: March 3,
2005 - Confidential annex added on March 8, 2005
Source:
http://gmoinfo.jrc.it/gmp_browse_geninf.asp
Notification
report
General information
Notification Number: B/ES/05/06-CON
Member State:Spain
Date of Acknowledgement:
Title of the Project: Official trials for registration
of maize varieties resistant to certain Lepidopteran insect
pests.
Proposed period of release From:01/04/2005
To:30/11/2005
Name of the Institute(s) or Company(ies): Dow
AgroSciences;
3. Is the same GMPt release planned elsewhere in the
Community?
Not known
4 - Has the same GMPt been notified elsewhere by the same
notifier?
No
Genetically
modified plant
1. Complete name of the
recipient or parental plant(s)
|
Common Name
|
Family Name
|
Genus |
Species
|
Subspecies
|
Cultivar/breeding line
|
| maize
|
poaceae |
zea
|
zea
mays |
mays
|
|
2. Description of the traits and characteristics which have
been introduced or modified, including marker genes and previous
modifications:
The cry1F(synpro) and pat genes were inserted into the maize
genome to express the Cry1F and PAT proteins, aiming at
conferring resistance to certain lepidopteran insect pests and
tolerance to glufosinate ammonium herbicide into the maize
plants.
Genetic
modification
3. Type of genetic
modification:
Insertion;
4. In case of insertion of genetic material, give the source
and intended function of each constituent fragment of the region
to be inserted:
The genetic material in PHI8999A intended to be inserted into
the maize genome contains a synthetic version of the cry1F and
pat genes, along with the regulatory signals required for the
transcription and the expression of these genes into the plant
tissues.
The cry1F gene (1818 bp. Origin Bacillus thuringiensis subs.
aizawai) is driven by the UbiZm1 promoter (1986 bp, origin zea
maiz) followed by the transcription terminator sequence ORF25
PolyA (714 bp, origin Agrobacterium tumefasciens pTi15995). The
function of the Cry1F protein in 1507 maize is to confer
resistance against some lepidopteran insect pests including
Sesamia spp.
The pat gene (552 bp; origin: Streptomyces viridochromogenes
strain Tü494) is under the control of the CaMV35S promoter and
terminator (554 y 204 bp, respectively; origin: mosaic
cauliflower). The function of the PAT protein is to confer
tolerance to maize 1507 against glufosinate ammonium herbicide.
See annex 1: List of genetic elements and brief description of
the transformation method (Confidential Business Information)
6. Brief description of the method used for the genetic
modification:
The genes were introduced into the maize genome by using the
particle bombardment technique with the linear fragment
containing the genes cry1F and pat genes along with the
regulatory sequences for their expression (PHI8999A).
7. If the recipient or parental plant is a forest tree
species, describe ways and extent of dissemination and specific
factors affecting dissemination:
Not relevant.
Experimental
Release
1. Purpose of the release:
The purpose of the release is to determine the field efficacy
of the Cry1F and PAT proteins expressed in transgenic maize 1507
against some lepidopteran insect pests under natural infestation
conditions. The Cry proteins are known to be very specific
against certain lepidopteran insects and no effects on
non-target organisms are expected.
No changes on survivability, weediness or dissemination were
observed in the field trials performed during the years 2000 and
2001 in the US, Argentina, South Africa and Brazil when compared
with conventional maize varieties as a consequence of the
expression of Cry1F or PAT proteins in maize 1507.
2. Geographical location of the site:
Aragón:Zaragoza (2 locations), Luceni (Zaragoza),Lalueza
(Huesca)
Castilla La Mancha: Oropesa (Toledo) (1 location)
Castilla León: Fuentesauco (Zaragoza) (1 location)
Andalucía: La Puebla del Rio (Sevilla) (1 location)
Cataluña: Bell-Lloc (Lleida) (1 location)
Navarra: Milagro(1 location)
3. Size of the site (m2):
Aragón: 6600 m2
Castilla la Mancha: 1500 m2
Castilla León: 3000 m2
Andalucía : 1500 m2
Cataluña: 1500 m2
Navarra: 2054 m2
4. Relevant data regarding previous releases carried out with
the same GM-plant, if any, specifically related to the potential
environmental and human health impacts from the release:
During the field trials performed in the US, Argentina,
Brazil, South Africa, no particular characteristics that could
have a negative impact on the environment or human health were
observed. Moreover, no significant differences between the maize
1507 and the conventional maize plants were detected.
Environmental
Impact and Risk Management
Summary of the potential
environmental impact from the release of the GMPts:
No maize sexual compatible species have been found in Spain.
Additionally, the field trials will be isolated from any other
maize field by at least 60 m. Maize has a long history of safe
use and been extensively domesticated by humans. The genes
introduced into the maize 1507, cry1F and pat have a very
specific mode of action, the first one conferring resistance to
some lepidopteran insect pests and the latter conferring
tolerance to the ammonium glufosinate herbicide. With the
adoption of this technology the insecticide will be localised
within the transgenic plants, reducing the environmental impact
to surrounding areas and the farmers will reduce fuel costs and
soil compaction through a substantial reduction in the numbers
of applications required to control insect pests.
Brief description of any measures taken for the management of
risks:
The trials are going to be isolated from any other maize plot
by a minimum distance of 60m. A non-GMO maize 4 row border will
be planted and subsequently destroyed at the end of the
experiment. Strict biosafety practices will be implemented
according to the Junta de …. guidelines. The following year a
different crop will be planted in these lots to facilitate the
detection and destruction of any volunteer plant. All the plant
products from these trials will be manually or chemically
destroyed. Trained people will carry on the experiment and
follow up closely. Regular monitoring visits will be implemented
during the growing season to evaluate the performance and
looking for any unintended effects and in the following season
to control volunteers
Summary of foreseen field trial studies focused to gain new
data on environmental and human health impact from the release:
These field trials are implemented to assess the efficacy of
the Cry1F protein expressed in maize 1507 against certain
lepidopteran insect pests and the and PAT protein conferring
tolerance against glufosinate ammonium herbicide. According to
the results obtained in the trials, the commercial strategy will
be drawn. |
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