Date of publication: June 22,
2005
Source:
http://gmoinfo.jrc.it/gmp_browse_geninf.asp
Notification number:
B/ES/05/23
Member State: Spain
Date of Acknowledgement:03/06/2005
Title of the Project:
Sucrose synthase characterization and its use for production
of potato plants with increased starch levels.
Proposed period of release From:01/06/2005
To:30/09/2005
Name of the Institute(s) or Company(ies): Instituto de
Agrobiotecnología, Universidad Pública de Navarra/Consejo
Superior de Investigaciones Científicas;
3. Is the same GMPt release planned elsewhere in the
Community?
No
4 - Has the same GMPt been notified elsewhere by the same
notifier?
No
Genetically
modified plant
1. Complete name of the
recipient or parental plant(s)
|
Common Name
|
Family Name
|
Genus
|
Species
|
Subspecies
|
Cultivar/breeding line
|
|
potato |
solanaceae |
solanum |
solanum tuberosum
|
tuberosum |
Desirée |
2. Description of the traits and characteristics which have
been introduced or modified, including marker genes and previous
modifications:
SuSy overexpressing potato plants produced have a complete
potato Sus4 cDNA under the control of CaMV-35S-promoter . In
this construction the selection gen was NPTII (kanamicin
resistance).
Genetic
modification
3. Type of genetic
modification:
Insertion; Other:
pBIN35S-susy-Nos
4. In case of insertion of genetic material, give the source
and intended function of each constituent fragment of the region
to be inserted:
Gen: Sucrose synthase (complete Sus4 cDNA from potato,
2425bp, accession number AJ537575 )
Selection gen: Kanamicin resistance (gen NPTII)
Promotor genes: CaMV35S (cauliflower mosaic virus promoter)
Terminator: NOS (nopaline synthase gen from Agrobacterium)
6. Brief description of the method used for the genetic
modification:
Potato plants transformation was conducted using
Agrobacterium tumefaciens strain C58C1:GV2260 as described by
Rocha-Sosa et al (Rocha-Sosa M., Sonnewald U., Frommer W.B.,
Startmann M., Schell J., Willmitzer L. (1989) EMBO J. 8, 23-29).
Experimental
Release
1. Purpose of the
release:
SuSy over-expressing plants grown in green-house exhibited a
large increase of starch levels as compare with WT and no
pleiotrophic changes in photosynthetic parameters and maximun
catalytic activities of enzymes closely linked to starch
metabolism could be detected. In order to prove that these
results also occur in field grown potato plants we decided to
release this material. If results are as expected, these plants
could be of great agonomic interest (will allow to reduce field
area and to produce agroenergy).
2. Geographical location of the site:
Genetic modified potato plants will be released in Sartaguda
(Navarra-Spain). The Experimental Field, which belongs to the
Instituto Técnico de Gestión Agrícola (ITGA), a governmental
Institution, is located in a dry area (5-50 mm precipitation) in
south Navarra.
3. Size of the site (m2):
The experimental field has 19 ha. However we will be using
only 100 m2.
4. Relevant data regarding previous releases carried out with
the same GM-plant, if any, specifically related to the potential
environmental and human health impacts from the release:
There is not previous releases.
Environmental
Impact and Risk Management
Summary of the potential
environmental impact from the release of the GMPts:
The introduced traits- overexpression of Sucrose synthase gen
under the control of CaMV-35S promoter- are not expected to
confer any selective advantage either any effect on environment
when compared with control plants.
Brief description of any measures taken for the management of
risks:
- We will control that nobody other people´s to the
experimental field harvests any potato plant.
- The potato plants will be surrounded by sexually remote
plants.
- It will be destroyed , by autoclaving, all the vegetal
material that is not going to be used.
- Potato tubers will not be seeded on the same parcel the
following campaign.
Summary of foreseen field trial studies focused to gain new
data on environmental and human health impact from the release:
We will plant 420 potato tubers. We will work with 6 genetic
modified lines and the control plants (WT line). We will use 60
tubers of each line, divided in 3 subparcel of 20 tubers each.
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