Deliberate release into the E.U.
environment of GMOs for any other purposes than placing on the
market:
Multiplication and field testing of
transgenic potatoes altered in their carbohydrate metabolism,
resulting in amylose-free tubers - The Netherlands - AVEBE |
Date of publication: February 23,
2005
Source:
http://gmoinfo.jrc.it/gmp_browse_geninf.asp
Notification
report
General information
Notification Number: B/NL/04/04
Member State: Netherlands
Date of Acknowledgement: 03/09/2004
Title of the Project:
Multiplication and field testing of transgenic potatoes
altered in their carbohydrate metabolism, resulting in
amylose-free tubers.
Proposed period of release From:01/01/2004
To:31/12/2013
Name of the Institute(s) or Company(ies): AVEBE;
3. Is the same GMPt release planned elsewhere in the
Community?
Not known
4 - Has the same GMPt been notified elsewhere by the same
notifier?
Yes
If yes, notification number(s):
B/NL/03/04
Genetically
modified plant
1. Complete name of the
recipient or parental plant(s)
|
Common Name
|
Family Name
|
Genus |
Species
|
Subspecies
|
Cultivar/breeding line
|
| potato
|
solanaceae |
solanum |
solanum tuberosum |
tuberosum |
all
cultivars |
2. Description of the traits and characteristics which have
been introduced or modified, including marker genes and previous
modifications:
Potato with an increased starch production (anti-GBSS)
without any selection markers.
Genetic
modification
3. Type of genetic
modification:
Insertion;
4. In case of insertion of genetic material, give the source
and intended function of each constituent fragment of the region
to be inserted:
pKGBA50mf-IR1.1 contains between the borders:
KGZ-prom: (=GBSS) Tuber specific and sugar inducible promoter of
the KGZ gene from Solanum tuberosum;
1.1 kb of sense DNA sequences of the KGZ gene from Solanum
tuberosum encoding the N-terminus of the KGZ protein;
AS-KGZ: anti-sense strand of the KGZ gene from Solanum
tuberosum.
pIRMASmf contains between the borders:
KGZ-prom: (=GBSS) Tuber specific and sugar inducible promoter of
the KGZ gene from Solanum tuberosum;
M-GBSSI (antisense): 617 bp (bps. 1070-1687) of antisense strand
of KGZ cDNA from Solanum tuberosum;
M-GBSSI (sense): 761 bp (bps. 926-1687) of KGZ-cDNA from Solanum
tuberosum.
6. Brief description of the method used for the genetic
modification:
The genetic material is inserted by Agrobacterium tumefaciens
cq Agrobacterium rhizogenes mediated transformation. Prior to
the introduction on the fields the studied plant lines will be
checked for the absence of vector backbone sequences by PCR.
Only vector-free plants will be introduced in the field.
Experimental
Release
1. Purpose of the release:
Field testing and propagation of genetically modified
potatoes.
2. Geographical location of the site:
North-East part of the Netherlands
3. Size of the site (m2):
1) Maximum of 10 sites per year.
2) Maximum total area per year of 5 hectares per year.
4. Relevant data regarding previous releases carried out with
the same GM-plant, if any, specifically related to the potential
environmental and human health impacts from the release:
The same GM-plant is part of the B/NL/01/11 and B/NL/03/04.
No special potential environmental and human health impacts are
expected nor detected.
Environmental
Impact and Risk Management
Summary of the potential
environmental impact from the release of the GMPts:
Similar plants with the same new trait have been evaluated
extensively in previous field experiments. The experiments did
not reveal an altered sensitivity for frost for both the
genetically modifiied plants and the amylose-free mutants. It is
therefore not likely that the genetic modification will result
in an altered frost sensitivity.
The GMP's do not contain any other introduced gene. The
potential impact will therefore be limited to the trait of an
altered carbohydrate composition. Other potential impacts on the
environment are therefore considered to be extreme unlikely.
Brief description of any measures taken for the management of
risks:
Besides the inactivation of the waste, no specific measures
are envisaged. Volunteer plants will be removed in accordance
with good agricultural practice.
Before the genetically modified potato plants are released in
the environment, they will be checked for the absence of vector
backbone sequences. Only those plants without vector backbone
sequences will be used in the actual field trials.
Summary of foreseen field trial studies focused to gain new
data on environmental and human health impact from the release:
No effects are expected. The monitoring of the plants, as
also in traditional breeding, is to observe deviations compared
to the parent cultivars. In an area , the walks and first row of
non-GMO potatoes around the GMO field, the volunteers will be
observed. |
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