Deliberate release into the
environment of GMOs for any other purposes than placing on the
market
Potato starch with increased amylose
content - Plant Science Sweden AB |
Date of publication: November 25, 2004
Source:
http://gmoinfo.jrc.it/gmp_browse_geninf.asp
Notification
report
General information
Notification Number: B/SE/04/7944
Member State: Sweden
Date of Acknowledgement:17/11/2004
Title of the Project: Potato starch with increased
amylose content
Proposed period of release From:01/01/2005
To:31/12/2009
Name of the Institute(s) or Company(ies): Plant
Science Sweden AB;
3. Is the same GMPt release planned elsewhere in the
Community?
No
4 - Has the same GMPt been notified elsewhere by the same
notifier?
No
Genetically
modified plant
1. Complete name of the
recipient or parental plant(s)
|
Common Name
|
Family Name
|
Genus
|
Species
|
Subspecies
|
Cultivar/breeding line
|
|
potato |
solanaceae |
solanum |
solanum tuberosum
|
tuberosum |
P763 |
2. Description of the traits and characteristics which have
been introduced or modified, including marker genes and previous
modifications:
- inhibition of starch branching enzymes SBE1 and SBE2, which
yields a higher fraction of amylose in the starch through a
reduced branching of amylopectin
- marker gene nptII (kanamycin resistance) as selection gene to
identify transgenic cells in tissue culture
Genetic
modification
3. Type of genetic
modification:
Insertion;
4. In case of insertion of genetic material, give the source
and intended function of each constituent fragment of the region
to be inserted:
T-DNA borders, pTiT37, for incorporation into plant
chromosome.
NptII gene, Tn 5, kanamycin resistance in plant material.
Promoter from nopaline synthase gene, A. tumefaciens, and g7
polyadenylation sequence, A. tumefaciens, gene regulation.
sbe1 and sbe2 gene fragments in antisense orientation relative
to the promoter, S. tuberosum, inhibition of amylopectin
synthesis.
Regulated by gbss promoter, S. tuberosum, and polyadenylation
sequence from nopaline synthase gene, A. tumefaciens.
6. Brief description of the method used for the genetic
modification:
Plasmid DNA was introduced into the potato lines by
Agrobacterium-mediated gene transfer technology.
7. If the recipient or parental plant is a forest tree
species, describe ways and extent of dissemination and specific
factors affecting dissemination:
Not applicable
Experimental
Release
1. Purpose of the
release:
- Investigate expression and stability of modified traits
- Investigate the agricultural and industrial value of the
modified lines.
- Produce potato tubers for laboratory analyses.
- Produce seed potatoes for future trials.
2. Geographical location of the site:
Landskrona, Halmstad, Kristianstad, Vara, Skara, Alvesta and
Gotlands communities.
3. Size of the site (m2):
Max 10 000 m2 in total
4. Relevant data regarding previous releases carried out with
the same GM-plant, if any, specifically related to the potential
environmental and human health impacts from the release:
No adverse impacts on the environment or human health have
been recorded for the potato line in previous field trials in
Sweden since 2000.
Environmental
Impact and Risk Management
Summary of the potential
environmental impact from the release of the GMPts:
The genetically modified potato line show a shift of the
tuber starch composition to amylose. No difference regarding
persistence in agricultural habitats or invasiveness in natural
habitats compared to conventional potato varieties is expected.
Nor is it expected that a selective advantage or disadvantage is
conferred to the GM lines via the introduced traits. Due to the
measures to be taken during the release (distance to or absence
of cultivated and wild relatives) will the potential for gene
transfer virtually be excluded. Interactions of the GM potato
lines with non-target species and resulting effects will be
comparable to those of conventional potato varieties. No toxic
or allergenic effects are expected from elevated levels of
amylose, nor from the nptII gene. Measures in place under
current field trial practice will safeguard that all tubers and
plant material is properly managed, harvested, stored,
transported or disposed of to minimize contact to humans or
animals. No effects are expected on biogeochemical processes.
The GM potato lines will be cultivated under conventional
agricultural practices.
Brief description of any measures taken for the management of
risks:
- isolation distance: 20 m
- careful harvest to avoid volunteers
- transportation in specific vehicles, closed containers or
double sacks
- adjusted crop rotation: fallow field the following year, no
potato crop for one additional year
- destruction of waste material
Summary of foreseen field trial studies focused to gain new
data on environmental and human health impact from the release:
Not planned |
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