Deliberate release into the
environment of GMOs for any other purposes than placing on the
market
Potato with increased amylose content
and improved starch biosynthesis - Aachen University of
Technology (RWTH); Plant Science Sweden AB |
Date of publication: November 25, 2004
Source:
http://gmoinfo.jrc.it/gmp_browse_geninf.asp
Notification
report
General information
Notification Number: B/SE/04/7945
Member State: Sweden
Date of Acknowledgement:
Title of the Project:
Potato with increased amylose content and improved starch
biosynthesis
Proposed period of release From:01/01/2005
To:31/12/2009
Name of the Institute(s) or Company(ies): Aachen
University of Technology (RWTH); Plant Science Sweden AB;
3. Is the same GMPt release planned elsewhere in the
Community?
No
4 - Has the same GMPt been notified elsewhere by the same
notifier?
No
Genetically
modified plant
1. Complete name of the
recipient or parental plant(s)
|
Common Name
|
Family Name
|
Genus |
Species
|
Subspecies
|
Cultivar/breeding line
|
| potato
|
solanaceae |
solanum |
solanum tuberosum |
tuberosum |
P763,
P800, P817 |
2. Description of the traits and characteristics which have
been introduced or modified, including marker genes and previous
modifications:
- inhibition of starch branching enzymes SBE1 and SBE2, which
yields a higher fraction of amylose in the starch through a
reduced branching of amylopectin
- improvement of starch biosynthesis
- marker gene ahas (imidazolinone tolerance) as selection gene
to identify transgenic cells in tissue culture
Genetic
modification
3. Type of genetic
modification:
Insertion;
4. In case of insertion of genetic material, give the source
and intended function of each constituent fragment of the region
to be inserted:
T-DNA borders, pTiT37, for incorporation into plant
chromosome.
Recombinant ahas gene, A. thaliana, imidazolinone tolerance in
plant material.
Promoter and polyadenylation sequence from nopaline synthase
gene, A. tumefaciens, gene regulation.
Inverted repeat fragments of sbe1 and sbe2 genes, S. tuberosum,
inhibition of amylopectin synthesis.
Gene for improvement of starch biosynthesis, S. tuberosum,
Regulated by gbss promoters, S. tuberosum, and polyadenylation
sequences from nopaline synthase gene, A. tumefaciens.
6. Brief description of the method used for the genetic
modification:
Plasmid DNA was introduced into the potato lines by
Agrobacterium-mediated gene transfer technology.
7. If the recipient or parental plant is a forest tree
species, describe ways and extent of dissemination and specific
factors affecting dissemination:
Not applicable
Experimental
Release
1. Purpose of the release:
- Investigate expression and stability of modified traits.
- Investigate the agricultural and industrial value of the
modified lines.
- Produce potato tubers for laboratory analyses.
- Produce seed potatoes for future trials.
2. Geographical location of the site:
Landskrona, Halmstad, Kristianstad, Gotland, Alvesta, Skara,
Vara, Luleå and Robertsfors communities.
3. Size of the site (m2):
Max 50 000 m2 in total
4. Relevant data regarding previous releases carried out with
the same GM-plant, if any, specifically related to the potential
environmental and human health impacts from the release:
No adverse impacts on the environment or human health have
been recorded for other potato lines with ahas gene in field
trials in Sweden since 2002, for potato lines with increased
amylose content since 1998 or potato lines with improved starch
biosynthesis since 2003.
Environmental
Impact and Risk Management
Summary of the potential
environmental impact from the release of the GMPts:
The genetically modified potato lines show a shift of the
tuber starch composition to amylose. No difference regarding
persistence in agricultural habitats or invasiveness in natural
habitats compared to conventional potato varieties is expected.
Nor is it expected that a selective advantage or disadvantage is
conferred to the GM lines via the introduced traits. Due to the
measures to be taken during the release (distance to or absence
of cultivated and wild relatives) will the potential for gene
transfer virtually be excluded. Interactions of the GM potato
lines with non-target species and resulting effects will be
comparable to those of conventional potato varieties. No toxic
or allergenic effects are expected from elevated levels of
amylose, nor from the ahas gene. Measures in place under current
field trial practice will safeguard that all tuber and plant
material is properly managed, harvested, stored, transported or
disposed of to minimize contact to humans or animals. No effects
are expected on biogeochemical processes. The GM potato lines
will be cultivated under conventional agricultural practices.
Brief description of any measures taken for the management of
risks:
- isolation distance: 20 m for trials and according to the
seed production regulation for seed production
- careful harvest to avoid volunteers
- transportation in specific vehicles, closed containers or
double sacks
- adjusted crop rotation: trials: fallow field the following
year, no potato crop for one additional year; seed production:
no potato crop for the following two years
- destruction of waste material
Summary of foreseen field trial studies focused to gain new
data on environmental and human health impact from the release:
Not planned |
|
|
|
