Date of publication: May 27, 2004
Source:
http://gmoinfo.jrc.it/gmp_browse_geninf.asp
Notification
report
General information
Notification Number:
B/IT/04/02
Member State: Italy
Date of Acknowledgement: 03/05/2004
Title of the Project:
Study of the stability of the transgene and his
hereditability of genetically modified wheat under of open field
conditions.
Proposed period of release From: 01/11/2004
To: 31/07/2005
Name of the Institute(s) or Company(ies): Metapontum
Agrobios s.c.a r.l.;
3. Is the same GMPt release planned elsewhere in the
Community?
No
4 - Has the same GMPt been notified elsewhere by the same
notifier?
No
Genetically
modified plant
1. Complete name of the
recipient or parental plant(s)
|
Common Name |
Family Name |
Genus |
Species |
Subspecies |
Cultivar/breeding line |
| spring
wheat |
poaceae
|
triticum
|
triticum
aestivum |
|
Bob white
|
2. Description of the traits and characteristics which have
been introduced or modified, including marker genes and previous
modifications:
From a genetic point of view, modifications brought to GMPt
consist in the insertion within the genomic DNA of the following
transgenes:
- Gene for the sub-unit Dx5B: fragment EcoRI of 8.7 Kb that
contain the gene Glu-D1-1b under transcriptional control of the
endogenous promoter for tissue-specific expression in wheat
endosperm;
- Gene for the sub-unity Dy10A: EcoRI fragment of 6.4 Kb that
contain the gene Glu-D1-2b under transcriptional control of the
endogenous promoter for tissue-specific expression in wheat
endosperm;
- Gene for the sub-unity Ax2: contain the gene Glu-A1-1c under
transcriptional control of the endogenous promoter for
tissue-specific expression in wheat endosperm
Genetic
modification
3. Type of genetic
modification:
Insertion;
4. In case of insertion of genetic material, give the source
and intended function of each constituent fragment of the region
to be inserted:
Organism donor: Triticum aestivum var Bob white
The sub-unity Dx5B, Dy10A e Ax2* have been inserted at the aim
to obtain an increasing level of gluteninic complex in wheat
endosperm.
The ubiquitin-BAR cassette has been inserted to allow the
selection of transformation events.
6. Brief description of the method used for the genetic
modification:
Transformants were obtained by bombardment using particle gun
Experimental
Release
1. Purpose of the release:
- Studies of stability of the transgenes;
- heredity in conditions of open field;
- evaluation of the agronomic performances of genetically
transformed wheat
2. Geographical location of the site:
The trial will be located in a field property of Metapontum
Agrobios s.c.a r.l, S.S. Jonica 106, Km 448.2, Metaponto (MT),
ITALY
3. Size of the site (m2):
5000 m2
4. Relevant data regarding previous releases carried out with
the same GM-plant, if any, specifically related to the potential
environmental and human health impacts from the release:
No previous release were carried out with this GM-plant.
Environmental
Impact and Risk Management
Summary of the potential
environmental impact from the release of the GMPts:
No unique factor to this field test has been identified that
would have an effect on any plant or animal species. In these
plants, there were no intentional changes influencing their
susceptibility to disease-causing organisms. Moreover there are
no reasons to believe that the GMPt peculiarity affects the
dissemination and the palatability of seeds to insects.
Cross-pollination with wild species is not possible since, up to
date, no interspecific crosses have been reported. Crosses of
the transgenic pollen with wheat commercial varieties is highly
unlikely since wheat show an high percentage of self-pollination
(95%). Moreover transgenic plot will be surrounded by wheat and
canola barriers and is fairly distant from other wheat fields.
Brief description of any measures taken for the management of
risks:
The field trial will take place on one small plot on
agricultural land in the Basilicata (ITALY). The site will
provide adequate physical security. Site monitoring of the field
trial and agronomic management practices creating controlled
environment are expected to provide the necessary degree of both
biological and physical containment. Pollen dispersal will be
avoided by canola and wheat barriers, which surround the entire
field. After sample collection and seed harvesting, the
remaining plants will be removed, autoclaved and/or burned and
finally disposed off. Possible plant residues left on the field
will be harvested and burned on site. The test area will be
marked to monitor re?emergence of volunteer wheat plants during
the following seasons. The plots will not be planted the
following season but will be checked several times to destroy
any plant material.
Summary of foreseen field trial studies focused to gain new
data on environmental and human health impact from the release:
The GMPt under discussion does not possess any form of
resistance or tolerance to pathogens or insects, so it does not
have any selective advantage respect to the control plant.
|
