Date of publication: March 19, 2004
Source:
http://gmoinfo.jrc.it/gmp_browse_geninf.asp
Notification
report
General information
Notification Number:
B/SE/04/1101
Member State:Sweden
Date of Acknowledgement:16/02/2004
Title of the Project:
Potato starch with increased amylose content
Proposed period of release From:01/04/2004
To:31/10/2008
Name of the Institute(s) or Company(ies): Plant
Science Sweden AB;
3. Is the same GMPt release planned elsewhere in the
Community?
No
4 - Has the same GMPt been notified elsewhere by the same
notifier?
No
Genetically
modified plant
1. Complete name of the
recipient or parental plant(s)
|
Common Name |
Family Name |
Genus |
Species |
Subspecies |
Cultivar/breeding line |
| potato
|
solanaceae
|
solanum
|
solanum
tuberosum |
tuberosum
|
P763 and
P800 |
2. Description of the traits and characteristics which have
been introduced or modified, including marker genes and previous
modifications:
- inhibition of starch branching enzymes be1 and be2, which
yields a higher fraction of amylose in the starch through a
reduced branching of amylopectin
- Marker gene AHAS (imidazolinone tolerance) as selection gene
to identify transgenic cells in tissue culture
Genetic
modification
3. Type of genetic
modification:
Insertion;
4. In case of insertion of genetic material, give the source
and intended function of each constituent fragment of the region
to be inserted:
T-DNA borders, pTiT37, for incorporation into plant
chromosome.Recombinant AHAS gene, A. thaliana, imidazolinone
tolerance in plant material.Promoter and polyadenylation
sequence from nopaline synthase gene, A. tumefaciens, gene
regulation. Inverted repeat fragments of be1 and be2, S.
tuberosum, inhibition of amylopectin biosynthesis.GBSS promoter,
S. tuberosum, expression of inverted repeat fragments.
6. Brief description of the method used for the genetic
modification:
A. tumefaciens mediated transformation of cut leaf discs from
S. tuberosum.Binary vector system has been used.Transgenic
tissue has been selected on medium containing imazamox. A.
tumefaciens has been killed with Claforan.
7. If the recipient or parental plant is a forest tree
species, describe ways and extent of dissemination and specific
factors affecting dissemination:
Not applicable
Experimental
Release
1. Purpose of the release:
- Investigate expression and stability of modified traits
- Identify and eliminate morphologically divergent clones
- Investigate the agricultural and industrial value of the
modified lines
- Produce potato tubers for laboratory analyses
- Produce seed potatoes for future trials
2. Geographical location of the site:
Landskrona, Halmstad, Kristianstad, Alvesta, Skara, Luleå och
Robertsfors communities
3. Size of the site (m2):
Max 50 000 m2 in total
4. Relevant data regarding previous releases carried out with
the same GM-plant, if any, specifically related to the potential
environmental and human health impacts from the release:
Modified potato lines with AHAS as marker gene (imazamox
tolerance gene) grown in field test 2002 and 2003.
Modified potato lines with inhibition of starch branching
enzymes and thus increased amylose content in field tests yearly
1998-2003.
No impact on the environment or human health has been observed
during these trials.
Environmental
Impact and Risk Management
Summary of the potential
environmental impact from the release of the GMPts:
There is no reason to assume that the transgenic lines will
behave in and interact with the environment differently compared
to their mother varieties.
There are no experiences that imply that an altered carbohydrate
composition or carbohydrate content could influence frost
tolerance neither of plants nor tubers. Neither high starch
content (starch potatoes) nor altered sugar content/composition
(chip potatoes) have such effects. Earlier genetic modifications
with increased amylose content have not been shown to influence
the frost tolerance.
The introduced selection marker gene gives an increased
tolerance to herbicides of imidazolinone type. This gives no
selective advantage compared to other potatoes outside the
agricultural habitat. As herbicides of imidazolinone type are
not used in Sweden no selective advantage in cultivated fields
is given either.
Brief description of any measures taken for the management of
risks:
- isolation distance: new transformants: 20 m
- careful harvest to avoid volunteers
- transportation in specific vehicles, closed containers or
double sacks
- adjusted crop rotation: new transformants: fallow field the
following year, no potato crop for one additional year
- destruction of waste material
Summary of foreseen field trial studies focused to gain new
data on environmental and human health impact from the release:
Not applicable |
