Date of publication: March 4, 2004
Source:
http://gmoinfo.jrc.it/gmp_browse_geninf.asp
Notification
report
General information
Notification Number:
B/NL/03/11
Member State:Netherlands
Date of Acknowledgement:10/11/2003
Title of the Project: Application for the release into
the environment of potato event AM99-2003.
Proposed period of release From:01/04/2004
To:31/12/2008
Name of the Institute(s) or Company(ies): BASF Plant
Science GmbH;
3. Is the same GMPt release planned elsewhere in the
Community?
Yes: Germany; Sweden
4 - Has the same GMPt been notified elsewhere by the same
notifier?
Yes
If yes, notification number(s): -
Genetically
modified plant
1. Complete name of the
recipient or parental plant(s)
|
Common Name |
Family Name |
Genus |
Species |
Subspecies |
Cultivar/breeding line |
| potato
|
solanaceae
|
solanum
|
solanum
tuberosum |
tuberosum
|
Dinamo
|
2. Description of the traits and characteristics which have
been introduced or modified, including marker genes and previous
modifications:
Genes modifying the starch composition in potato tubers
raising the level of amylose have been introduced into potato.
The neomycin phosphotransferase II gene has been used as
selectable marker gene.
Genetic
modification
3. Type of genetic
modification:
Insertion;
4. In case of insertion of genetic material, give the source
and intended function of each constituent fragment of the region
to be inserted:
In potato line AM99-2003 a gene consisting pf gbss promoter
from Solanum tuberosum, the coding region fragments of be1 and
be2 (starch-branching enzyme) in tandem and antisense
orientation relative to the promoter and the nos polyadenylation
sequence from Agrobacterium tumefaciens have been inserted into
potato variety Dinamo thus reducing the amount of amylopectin in
the starch fraction of the tuber. In addition the neomycin
phosphotransferase gene (nptII) connected to the Agrobacterium
tumefaciens nopaline synthase promoter and g7 polyadenylation
sequence from Agrobacterium tumefaciens nopaline as selectable
marker gene conferring resistance to kanamycin.
6. Brief description of the method used for the genetic
modification:
Plasmid DNA was introduced into the potato line by
Agrobacterium-mediated gene transfer technology. This is
standard technology for potato transformation.
Experimental
Release
1. Purpose of the release:
The purpose of the release is, within the frame of safety
studies for event AM99-2003, to compile data on agronomical
performance and environmental effects, as well as to collect
plant material for further analyses (e.g. biochemistry,
molecular biology). It is anticipated that in part the trial
will be used as well to generate seed tubers.
2. Geographical location of the site:
The location of the release site will be at Borger-Odoorn,
the Netherlands.
3. Size of the site (m2):
1 ha.
4. Relevant data regarding previous releases carried out with
the same GM-plant, if any, specifically related to the potential
environmental and human health impacts from the release:
No adverse impacts on the environment or human health have
been recorded for the line AM99-2003 in previous field trials in
Sweden.
Environmental
Impact and Risk Management
Summary of the potential
environmental impact from the release of the GMPts:
The genetically modified potato line shows a shift of the
tuber starch composition to amylose. No difference regarding
persistence in agricultural habitats or invasiveness in natural
habitats compared to conventional potato varieties is expected.
Nor is it expected that a selective advantage or disadvantage is
conferred to the GM line via the introduced traits. Due to the
measures to be taken during the release (distance to or absence
of cultivated and wild relatives) will the potential for gene
transfer virtually excluded. Interactions of the GM potato line
with non-target species and resulting effects will be comparable
to those of conventional potato varieties. No toxic or
allergenic effects are expected from elevated levels of amylose
starch, nor from the neomycin phosphotransferase. Measures in
place under current field trial practice will protect the trial
from damage by animals as well as safeguard that all seed and
plant material is properly managed, harvested, stored,
transported or disposed of to minimize or prevent contact to
humans or animals. No effects are expected on biogeochemical
processes. The GM potato lines will be cultivated under
conventional agricultural practices.
Brief description of any measures taken for the management of
risks:
An isolation distance of 20 m to other potato varieties will
be observed. Planting and harvesting equipment will be cleaned
in site to prevent the dispersal of GM tubers. There will be no
potato cultivation on the release area for the year following
the release. Potential volunteers be monitored and removed
according to conventional agricultural practice. During the
release the trial site will be monitored at defined intervals.
|
