Deliberate release into the
environment of GMOs for any other purposes than placing on the
market
Application for the release into the
environment of potato events AM02-1003, AM01-1005, AM02-1012,
AM02-1017, AM99-1089 and AM99-2003 - BASF Plant Science GmbH |
Date of publication: February 5, 2004
Source:
http://gmoinfo.jrc.it/gmp_browse_geninf.asp
Notification
report
General information
Notification Number:
B/DE/03/154
Member State:Germany
Date of Acknowledgement:17/11/2003
Title of the Project:
Application for the release into the environment of potato
events AM02-1003, AM01-1005, AM02-1012, AM02-1017, AM99-1089 and
AM99-2003.
Proposed period of release From:01/04/2004
To:31/10/2008
Name of the Institute(s) or Company(ies): BASF Plant
Science GmbH;
3. Is the same GMPt release planned elsewhere in the
Community?
Yes: Netherlands; Sweden
4 - Has the same GMPt been notified elsewhere by the same
notifier?
Yes
If yes, notification number(s):
-
Genetically
modified plant
1. Complete name of the
recipient or parental plant(s)
|
Common Name |
Family Name |
Genus |
Species |
Subspecies |
Cultivar/breeding line |
| potato
|
solanaceae
|
solanum
|
solanum
tuberosum |
tuberosum
|
Seresta,
Kuras, Dinamo. |
2. Description of the traits and characteristics which have
been introduced or modified, including marker genes and previous
modifications:
- A gene modifying the starch composition in potato tubers
raising the level of amylopectin has been introduced into
potato. The neomycin phosphotransferase II gene or an
acetohydroxyacid synthase gene have been used as selectable
marker genes.
- Genes modifying the starch composition in potato tubers
raising the level of amylose have been introduced into potato.
The neomycin phosphotransferase II gene has been used as
selectable marker gene.
Genetic
modification
3. Type of genetic
modification:
Insertion;
4. In case of insertion of genetic material, give the source
and intended function of each constituent fragment of the region
to be inserted:
a) A gene containing the coding region of potato gbss in
antisense orientation relative to the promoter, flanked by the
gbss promoter from Solanum tuberosum and the polyadenylation
sequence from Agrobacterium tumefaciens nopaline synthase gene
has been inserted into potato variety Seresta (lines AM02-1003,
AM01-1005, AM02-1012) and Kuras (line AM02-1017) thus reducing
the amount of amylose in the starch fraction. An ahas gene
(acetohydroxyacid synthase) from Arabidopsis thaliana flanked by
the nos gene promoter and the octopine synthase polyadenylation
sequence from Agrobacterium tumefaciens serves as selectable
marker gene conferring tolerance to Imazamox.
b) AM99-1089 serves as a reference line. The inserted gene
consists of the potato gbss (granule bound starch synthase)
promoter, the coding region of potato gbss in antisense
orientation and the polyadenylation sequence from Agrobacterium
tumefaciens nopaline synthase gene thus reducing the amount of
amylose in the starch fraction. In addition the neomycin
phosphotransferase gene (nptII) connected to the Agrobacterium
tumefaciens nopaline synthase promoter and g7 polyadenylation
sequence from Agrobacterium tumefaciens has been inserted as
selectable marker gene conferring resistance to kanamycin.
c) In potato line AM99-2003 a gene consisting of gbss promoter
from Solanum tuberosum, the coding region fragments of be1 and
be2 (starch-branching enzyme) in tandem and antisense
orientation relative to the promoter and the nos polyadenylation
sequence from Agrobacterium tumefaciens have been inserted into
potato variety Dinamo thus reducing the amount of amylopectin in
the starch fraction of the tuber. In addition the neomycin
phosphotransferase gene (nptII) connected to the Agrobacterium
tumefaciens nopaline synthase promoter and g7 polyadenylation
sequence from Agrobacterium tumefaciens has been inserted as
selectable marker gene conferring resistance to kanamycin.
6. Brief description of the method used for the genetic
modification:
Plasmid DNA was introduced into the potato lines by
Agrobacterium-mediated gene transfer technology. This is
standard technology for potato transformation.
7. If the recipient or parental plant is a forest tree
species, describe ways and extent of dissemination and specific
factors affecting dissemination:
Not applicable.
Experimental
Release
1. Purpose of the release:
The purpose of the release is, within the frame of safety
studies for events AM02-1003, AM02-1005, AM02-1012, AM02-1017,
AM99-1089 (as a comparator high amylopectin event) and
AM99-2003, to compile data on agronomical performance and
environmental effects, as well as to collect plant material for
further analyses (e.g. biochemistry, molecular biology). It is
anticipated that in part the trial will be used as well to
generate seed tubers.
2. Geographical location of the site:
The location of the release site will be in Sachsen-Anhalt
and Bavaria.
3. Size of the site (m2):
8.000 m2 and 9.000 m2.
4. Relevant data regarding previous releases carried out with
the same GM-plant, if any, specifically related to the potential
environmental and human health impacts from the release:
No adverse impacts on the environment or human health have
been recorded for the lines AM02-1003, AM02-1005, AM02-1012,
AM02-1017, AM99-1089 and AM99-2003 in previous field trials in
Sweden.
Environmental
Impact and Risk Management
Summary of the potential
environmental impact from the release of the GMPts:
The genetically modified potato lines show a shift of the
tuber starch composition to amylopectin or amylose,
respectively. No difference regarding persistence in
agricultural habitats or invasiveness in natural habitats
compared to conventional potato varieties is expected. Nor is it
expected that a selective advantage or disadvantage is conferred
to the GM lines via the introduced traits. Due to the measures
to be taken during the release (distance to or absence f
cultivated and wild relatives) will the potential for gene
transfer virtually excluded. Interactions of the GM potato lines
with non-target species and resulting effects will be comparable
to those of conventional potato varieties. No toxic or
allergenic effects are phosphotransferase or acetohydroxyacid
synthase. Measures in place under current field trial practice
will protect the trial from damage by animals as well as
safeguard that all seed and plant material is properly managed,
harvetsed, stored, transported or disposed of to minimise or
prevent contact to humans or animals. No effects are expected on
biogeochemical processes. The GM potato lines will be cultivated
under conventional agricultural practices.
Brief description of any measures taken for the management of
risks:
An isolation distance of 10 m to other potato varieties will
be observed. Planting and harvesting equipment will be cleaned
on site to prevent the dispersal of GM tubers. There will be no
potato cultivation on the release area the year following the
release. Potential volunteers will be monitored and removed
according to conventional agricultural practice. During the
release the trial site will be monitored at defined intervals.
Summary of foreseen field trial studies focused to gain new
data on environmental and human health impact from the release:
Not applicable. |
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