Date of publication: April 1, 2004
Source:
http://gmoinfo.jrc.it/gmp_browse_geninf.asp
Notification
report
General information
Notification Number:
B/ES/04/12
Member State:Spain
Date of Acknowledgement: 25/02/2004
Title of the Project: Testing of maize varieties
tolerant to an herbicide
Proposed period of release From: 01/04/2004
To:30/11/2004
Name of the Institute(s) or Company(ies): PIONEER
HI-BRED SPAIN S.L.;
3. Is the same GMPt release planned elsewhere in the
Community?
No
4 - Has the same GMPt been notified elsewhere by the same
notifier?
No
Genetically
modified plant
1. Complete name of the
recipient or parental plant(s)
|
Common Name |
Family Name |
Genus |
Species |
Subspecies |
Cultivar/breeding line |
| maize
|
poaceae
|
zea
|
zea mays
|
mays
|
Experimental hybrids and inbred lines |
2. Description of the traits and characteristics which have
been introduced or modified, including marker genes and previous
modifications:
One transformation event, coded NK603, will be used for the
proposed field programme. The maize plants express a CP4 EPSPS
gene which confers tolerance to glyphosate, the active
ingredient of Roundup herbicide. There have been no previous
genetic modifications of the parental organism.
Genetic
modification
3. Type of genetic
modification:
Insertion;
4. In case of insertion of genetic material, give the source
and intended function of each constituent fragment of the region
to be inserted:
The donor organism, Agrobacterium sp. strain CP4, was used to
supply the CP4 EPSPS gene for glyphosate tolerance. Genetic
analysis of line NK603 confirmed a single stable site of
insertion of the sequences encoding CP4 EPSPS into the maize
plant genome. The insertion resulted in the expression of the
closely related proteins CP4 EPSPS and CP4 EPSPS L214P, which
imparts tolerance to glyphosate.
6. Brief description of the method used for the genetic
modification:
The transformation vector, containing the CP4 EPSPS gene and
regulatory sequences, was introduced into the maize genome by a
particle acceleration method to produce Roundup Ready maize line
NK603.
7. If the recipient or parental plant is a forest tree
species, describe ways and extent of dissemination and specific
factors affecting dissemination:
Not applicable.
Experimental
Release
1. Purpose of the release:
The goal of the release is the testing of genetically
modified maize varieties in view of collecting regulatory data
for registration on the National Catalogue.
2. Geographical location of the site:
The release is planned at one location in Spain in 2004:
- Andalucía: La Rinconada (Sevilla)
3. Size of the site (m2):
The trial at the site in 2004 will cover about 514 m² (all
varieties and borders included) of which 90 m² will be planted
with the transgenic plants concerned by this application.
4. Relevant data regarding previous releases carried out with
the same GM-plant, if any, specifically related to the potential
environmental and human health impacts from the release:
Field trials were conducted in several locations and no
environmental problems were reported for these trials. The
transgenic plants behaved as conventional maize. They were
indistinguishable from the non-genetically modified maize plants
except for showing herbicide tolerance when glyphosate is
applied.
Environmental
Impact and Risk Management
Summary of the potential
environmental impact from the release of the GMPts:
No risks to human and animal health or the environment from
the deliberate release of genetically modified maize tolerant to
glyphosate have been reported.
Several generations of the transgenic maize plants have been
tested previously in different environments in the US since 1997
and in Europe since 1999. These experiments have demonstrated
that the trait is stably inherited and that the plants resemble
the recipient plant in all respect differing only in their
ability to be tolerant to glyphosate. Measures will be taken to
avoid seed dissemination at harvest and during transportation
(see E.).
Maize can hybridise with teosinte (Zea mays ssp. Mexicana) but
these plants are not present in Europe. The possibility of
hybridisation with other maize plants will be minimised by a
spatial isolation distance of minimum 200 meters from other
cultivated.
Brief description of any measures taken for the management of
risks:
In order to limit any pollen flow from the genetically
modified plants, a 200m-isolation distance will be maintained to
any other commercial maize crop.
Seed dispersal of individual kernels does not generally occur.
They are fixed on a cob and enclosed in many husks that protect
the seeds from outside contact. For this trial, a few seeds will
need to be collected for analysis; this will be done by sampling
the whole ear and unused seeds will be destroyed.
In case of any emergency, the trial could be stopped by
application of a non-selective herbicide other than glyphosate
or by mechanical destruction and incorporation into the soil.
At the end of the release all remaining plant matter that has
not been harvested for analyses will be destroyed by chopping
and incorporating into the soil.
Volunteer maize will be monitored during the following year. An
herbicide treatment (other than glyphosate) will be used in
order to ensure destruction if needed.
Summary of foreseen field trial studies focused to gain new
data on environmental and human health impact from the release:
Not applicable to this release. |
