As mentioned
above, the insertion of advantageous traits has really
revolutionized the test methods and added a new dimension to
seed quality testing. Both the owner of the advantageous gene
and the seed producer have "quality requirements" that need to
be met. Millions of dollars have been spent to develop
products with an advantageous trait and protection of that
investment has resulted in highly specific tests and sampling
schemes. Also, consumer fears about the inserted gene have
resulted in yet completely different sampling scheme and
reason to test. How a seed testing company tests for "quality"
is quite different than how it may test for the "absence" of
the gene to appease consumer fears or meet import
requirements. You really have to pay attention to the clients
needs to determine what test method is best and how the seed
needs to be sampled.
In the case of seed health testing,
it is not really new seeds that have driven change, but the
changes in seed production and seed-user demands that have
revolutionized the technologies and protocols used to detect
seed-borne pathogens. The basic technology for seed health
testing has not changed much over the last few years, although
serology and PCR have increased in use, these techniques have
been applied to seed health methods for several years. What we
see changing is the size of samples being tested for
seed-borne pathogens and more precision in identifying the
pathogen in the test method to protect the seed producer from
liability risk. Seed production practices are more "healthy"
and there are rarely large infections of seed-borne pathogens
in seed fields, anymore. If a seed-borne pathogen does infect
a seed lot these days, it tends to be infecting less seed with
fewer numbers of the pathogen and it tends to be more
deep-seated or internal, thus more difficult to detect.
Increasing sample sizes are one way to improve confidence in
the seed health test, as is using more tools, such as PCR.
As an example, it was only 2 years
ago that seed producers were testing 10,000 seeds for
bacterial fruit blotch (BFB), a highly contagious disease of
watermelon and cantaloupe. Routinely now, producers are
testing 30,000 to 50,000 seeds. The general grow out method
for BFB testing has not changed since its development in 1994.
It remains the standard for the industry and when done in
precision controlled greenhouses, it is quite reliable. Our
greenhouses maintain conditions that closely mimic the growing
environment of the Southeastern United States, where BFB
continues to cause problems in the melon industries. No test
can be 100% accurate, but the grow-out has proven to be more
than 99.9% accurate in the 8 years that STA has been
performing the test. The primary reasons that sample sizes
have increased 3-5 times in two years, is that BFB continues
to cause economic losses to greenhouse grown transplants,
where conditions for BFB are ideal at even the lowest levels
of infection and it continues to be a liability risk to the
seed producers. Also, overseas production of these
hand-pollinated crops in the driest of climates has created a
situation where symptoms of BFB are extremely difficult to
observe. Seed producers are therefore, relying on seed health
testing as their primary way to manage the risk of BFB seed
contamination for their customers.
The trend toward larger sample sizes
can be applied to most of the seed-borne pathogen tests for
most vegetable and flower crops, but we also find that with
seed lots produced overseas, some organisms recovered from a
seed health test, may look like the pathogen, but not cause
plant disease. In these cases, we have found more and more
identification tools, such as PCR and ELISA, to help us
distinguish the pathogen from these look-alike organisms. We
have also had to apply more quality assurance standards to our
seed health testing program, to document test validity. We
routinely include several strains as positive checks in many
of our seed health tests, for example.