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SHANKAR VINJAMURI
H.No. 3-5-937, Susheelwadi
Himayatnagar, Hyderabad 29
India
Ph.: 9849019683, 9849742543
E-mail:
shankarv_73@rediffmail.com
RESEARCH PROFESSIONAL - PLANT MOLECULAR BIOLOGY / GENETIC ENGG
M.Sc. (Botany)/Established credibility as a focused and
dependable performer in conducting research projects
JOB OBJECTIVE: Seeking an assignment as a 'scientist' in
a progressive company or research organization in India/ abroad.
PROFILE
-
10 years
of career success in conducting productive research in the
field of Plant Molecular Biology /Genetic Engineering.
-
Core
competencies in project planning, designing and executing
biotechnological research projects. Proven expertise in
writing research reports and making technical presentation.
-
Hold
credentials of publishing several abstract research papers.
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Progressive experience in genetic transformation of crops
like Sorghum, Maize, Cotton, Brinjal, Brassica, Pigeon pea,
Watermelon through Agrobacterium and Particle Bombardment
(PIG) and Molecular characterization of Transgenic.
-
Acquired
professional expertise in handling various equipments and
lab Instruments like PCR, AFLP Units, GEL Documentation,
Spectro Photo Meter, Electrophoresis.
-
Proficient in effective application of research techniques
and Analytical Methods like × DNA Finger Printing ×
Identifying Molecular Markers × Southern Hybridization &
Microbiology Techniques like Maintenance Plasmids ×
Cryogenic Preservations and general Molecular Biology
Techniques.
-
Self-driven, motivated and work focused with superior
written and verbal communication skills.
PROFESSIONAL EXPERIENCE
Jan 2006
-Feb 2008
Bioseed Research India Pvt Ltd
Research executive
Dec 2002 - Dec 2005
J.K Agri Genetics Ltd
Research Associate
Jul 1998 – Nov 2002
National Research Centre for Sorghum (NRCS), an ICAR
Institute, GOI
Research Associate
Jun 1997– Jun 1998
Mahyco Research Foundation
Research Assistant
Short Duration
Directorate of Rice Research (DRR), an ICAR
Institute, GOI
Graduate Assistant
Major
Projects Handled
Bioseed Research India pvt Ltd.:
Project: Tissue culture and genetic transformation of crops
like Cotton, Maize and Pigeon pea.
Role: Research Executive
Description: The research approach followed was
Agrobacterium mediated genetic transformation. The
activities performed work included development of suitable
protocol for Agrobacterium mediated transformation,
standardization of Gus gene expression and PCR analysis of
putative transformants.
J.K.Agri genetics Ltd:
Project: Tissue culture and genetic transformation of crops
like Cotton, Sorghum, Watermelon Brinjal and Brassica.
Role: Research Associate
Description: The approach followed was through Particle
Bombardment (PIG) & Agrobacterium mediated genetic
transformation. The work profile included × Development of
suitable protocol for Agrobacterium method × Standardization
of Gus gene expression × Regeneration of calli (under
progress) × PCR analysis of putative transformants.
National Research Centre for Sorghum (NRCS)
Project: Evolving transgenic sorghum with suitable Bt Gene
resistant to Stem borer.
Role: Research Associate
Description: The methodology involved Particle Bombardment &
Agrobacterium mediated genetic transformation. Activities
performed covered development of suitable protocol for
Agrobacterium method, Standardization of Gus gene expression
and regeneration of calli. PCR analysis of putative
transformants was done with suitable Gus & Hygromycin
primers (60% were confirmed to be positive).
Achievements:
×Standardization of transformation protocol with reporter
gene (gus) gene thru' histochemical assay.
×Southern hybridization with suitable probe for the
conformation of stable integration of cry1Ac genes
Mahyco Research Foundation
Project- I: Randomly Amplified Polymorphic DNA (RAPD) Finger
Printing studies of A, B, R lines to identify different
source of CMS lines from Wild Abortive (WA) cytoplasm in
Rice (Oryza sativa).
Role: Research Assistant
Description: The work involved Mitochondria DNA Isolation &
Finger Printing
Achievements:
× Mitochondrial DNA Polymorphic studies were conducted
between various wild genotypes & observed polymorphism using
RAPD technique.
Project - II: Molecular tagging of BPH (Brown plant hopper)
resistant gene & marker assisted selection (MAS) for insect
resistance in Rice (Oryza sativa).
Role: Research Assistant
Description: DNA restriction analysis, Southern
hybridization of double haploid population of IR 64 x
Azucena. Over 150 F1 & 70 F2 plants were analyzed in the
studies.
Achievements:
× Identified Marker (0.98Kb) gene having tight linkage,
which confer resistance to BPH in rice.
PUBLICATIONS:
•S.V. Rao, K.B.R.S. Visarada, D. Balakrishna, V. Shankar.
Presented a Poster at “88th session of the Indian Science
Congress” at IARI, New Delhi, and January 3-7, 2001. Title:
Agrobacterium Mediated Genetic Transformation of Sorghum.
•S.V. Rao, K.B.R.S. Visarada, D. Balakrishna, V. Shankar.
Presented a paper at National Symposium on Plant genetic
transformation at Directorate of Oilseeds Research, March 27-28,
2000. Title: Current status of genetic transformation in
sorghum.
•S.V. Rao, K.B.R.S. Visarada, D. Balakrishna, V. Shankar and B.
Srinivas.
Presented a paper at International Workshop on “Sorghum tissue
culture, transformation & genetic engineering” at ICRISAT, March
8-9, 1999.
•S.V. Rao, K.B.R.S. Visarada, D. Balakrishna, V. Shankar & B.
Srinivas.
Presented a paper at National Symposium on “Recent trends on
plant cytogenetics & biotechnology” at Andhra University, Feb.
21-22, 1999. Title: “Genetic transformation of post rainy season
sorghum genotypes using particle bombardment”.
•K.K. Jena, I.C. Pasalu, K. Krishnaiah, V. Shankar and Y. Kondal
Rao.
Presented a paper at ASIAN RICE BIOTECHNOLOGY NETWORK (ARBN)
Mini Network, April 1998 entitled, “Molecular tagging of BPH
resistant gene & MAS for insect resistant breeding in rice”.
EDUCATION
Master of Science (Botany)
Annamalai University, First Division.
Date of birth: 15.02.1973
Nationality: Indian
Marital status: Married
References: Available on request .
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